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A Small RNA Transforms the Multidrug Resistance of Pseudomonas aeruginosa to Drug Susceptibility
- Source :
- Molecular Therapy. Nucleic Acids, Molecular Therapy: Nucleic Acids, Vol 16, Iss, Pp 218-228 (2019)
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Bacteria with multiple drug resistance (MDR) have become a global issue worldwide, and hundreds of thousands of people’s lives are threatened every year. The emergence of novel MDR strains and insufficient development of new antimicrobial agents are the major reasons that limit the choice of antibiotics for the treatment of bacterial infection. Thus, preserving the clinical value of current antibiotics could be one of the effective approaches to resolve this problem. Here we identified numerous novel small RNAs that were downregulated in the MDR clinical isolates of Pseudomonas aeruginosa (P. aeru), and we demonstrated that overexpression of one of these small RNAs (sRNAs), AS1974, was able to transform the MDR clinical strain to drug hypersusceptibility. AS1974 is the master regulator to moderate the expression of several drug resistance pathways, including membrane transporters and biofilm-associated antibiotic-resistant genes, and its expression is regulated by the methylation sites located at the 5′ UTR of the gene. Our findings unravel the sRNA that regulates the MDR pathways in clinical isolates of P. aeru. Moreover, transforming bacterial drug resistance to hypersusceptibility using sRNA could be the potential approach for tackling MDR bacteria in the future.
- Subjects :
- 0301 basic medicine
Small RNA
biology
medicine.drug_class
Pseudomonas aeruginosa
lcsh:RM1-950
Antibiotics
Drug resistance
Antimicrobial
biology.organism_classification
medicine.disease_cause
Article
Microbiology
Multiple drug resistance
03 medical and health sciences
lcsh:Therapeutics. Pharmacology
030104 developmental biology
0302 clinical medicine
030220 oncology & carcinogenesis
Drug Discovery
medicine
Molecular Medicine
Gene
Bacteria
Subjects
Details
- ISSN :
- 21622531
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Molecular Therapy - Nucleic Acids
- Accession number :
- edsair.doi.dedup.....7e8b3588a1538eee33b7a301f7faf343