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Secretome Analysis of Mouse Dendritic Cells Interacting with a Probiotic Strain of Lactobacillus gasseri
- Source :
- Nutrients, Nutrients, Vol 12, Iss 2, p 555 (2020), Volume 12, Issue 2, Nutrients 12 (2020). doi:10.3390/nu12020555, info:cnr-pdr/source/autori:Mazzeo M.F.; Luongo D.; Sashihara T.; Rossi M.; Siciliano R.A./titolo:Secretome analysis of mouse dendritic cells interacting with a probiotic strain of lactobacillus gasseri/doi:10.3390%2Fnu12020555/rivista:Nutrients/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:12
- Publication Year :
- 2020
- Publisher :
- MDPI, 2020.
-
Abstract
- Probiotics play a key role in the modulation of the gut immune system in health and disease and their action is mediated by molecules exposed on the microorganism surface or secreted probiotic-derived factors. In particular, Lactobacillus gasseri OLL2809, a probiotic microorganism isolated from human feces, has the potential to modulate various immune responses. The dendritic cells (DCs) are considered the main players in orchestrating the immune response, and their contact with intestinal microbiota is crucial for the development and homeostasis of gut immunity. To gain a perspective on the molecular mechanisms involved in the maturation process of DCs and investigate factors that could modulate these processes, a differential proteomic analysis was performed on the secretome of immature DCs, mature DCs (mDCs, induced by lipopolysaccharide (LPS)), and immature DCs challenged with L. gasseri OLL2809 before treatment with LPS (LGmDCs). The maturation process of DCs was associated to profound changes in the protein secretome and probiotic pre-treatment led to a dramatic modulation of several secreted proteins of mDC, not only classical immune mediators (i.e., cytokines, complement factors, T cell Receptor ligands) but also proteins involved in the contractile and desmosome machineries. The latter data highlight a novel mechanism by which L. gasseri can modulate the maturation process of DCs, reinforcing the concept of a protective anti-inflammatory role ascribed to this probiotic strain.
- Subjects :
- 0301 basic medicine
Proteomics
Lipopolysaccharide
lcsh:TX341-641
chemical and pharmacologic phenomena
Lactobacillus gasseri
immunomodulation
Article
law.invention
secreted proteins
lactobacillus gasseri
03 medical and health sciences
chemistry.chemical_compound
Probiotic
Feces
Mice
0302 clinical medicine
Immune system
law
Animals
Immunologic Factors
Cellular Senescence
mass spectrometry
Nutrition and Dietetics
biology
Probiotics
T-cell receptor
Dendritic Cells
biology.organism_classification
Cell biology
030104 developmental biology
Secretory protein
chemistry
030220 oncology & carcinogenesis
lcsh:Nutrition. Foods and food supply
Homeostasis
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 20726643
- Volume :
- 12
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Nutrients
- Accession number :
- edsair.doi.dedup.....7eaa166c9e50de949dc4ef5cd44d6e47
- Full Text :
- https://doi.org/10.3390/nu12020555