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Secretome Analysis of Mouse Dendritic Cells Interacting with a Probiotic Strain of Lactobacillus gasseri

Authors :
Toshihiro Sashihara
Rosa Anna Siciliano
Mauro Rossi
Maria Fiorella Mazzeo
Diomira Luongo
Source :
Nutrients, Nutrients, Vol 12, Iss 2, p 555 (2020), Volume 12, Issue 2, Nutrients 12 (2020). doi:10.3390/nu12020555, info:cnr-pdr/source/autori:Mazzeo M.F.; Luongo D.; Sashihara T.; Rossi M.; Siciliano R.A./titolo:Secretome analysis of mouse dendritic cells interacting with a probiotic strain of lactobacillus gasseri/doi:10.3390%2Fnu12020555/rivista:Nutrients/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:12
Publication Year :
2020
Publisher :
MDPI, 2020.

Abstract

Probiotics play a key role in the modulation of the gut immune system in health and disease and their action is mediated by molecules exposed on the microorganism surface or secreted probiotic-derived factors. In particular, Lactobacillus gasseri OLL2809, a probiotic microorganism isolated from human feces, has the potential to modulate various immune responses. The dendritic cells (DCs) are considered the main players in orchestrating the immune response, and their contact with intestinal microbiota is crucial for the development and homeostasis of gut immunity. To gain a perspective on the molecular mechanisms involved in the maturation process of DCs and investigate factors that could modulate these processes, a differential proteomic analysis was performed on the secretome of immature DCs, mature DCs (mDCs, induced by lipopolysaccharide (LPS)), and immature DCs challenged with L. gasseri OLL2809 before treatment with LPS (LGmDCs). The maturation process of DCs was associated to profound changes in the protein secretome and probiotic pre-treatment led to a dramatic modulation of several secreted proteins of mDC, not only classical immune mediators (i.e., cytokines, complement factors, T cell Receptor ligands) but also proteins involved in the contractile and desmosome machineries. The latter data highlight a novel mechanism by which L. gasseri can modulate the maturation process of DCs, reinforcing the concept of a protective anti-inflammatory role ascribed to this probiotic strain.

Details

Language :
English
ISSN :
20726643
Volume :
12
Issue :
2
Database :
OpenAIRE
Journal :
Nutrients
Accession number :
edsair.doi.dedup.....7eaa166c9e50de949dc4ef5cd44d6e47
Full Text :
https://doi.org/10.3390/nu12020555