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The acetate recovery factor to correct tracer-derived dietary fat oxidation in humans
- Source :
- AJP-Endocrinology and Metabolism, AJP-Endocrinology and Metabolism, American Physiological Society, 2008, 294 (4), pp.E645-E653. ⟨10.1152/ajpendo.00720.2007⟩
- Publication Year :
- 2008
- Publisher :
- HAL CCSD, 2008.
-
Abstract
- When using 13C tracer to measure plasma fat oxidation, an acetate recovery factor should be determined in every subject to correct for label sequestration. Less is known regarding the acetate recovery factor for dietary fatty acid oxidation. We compiled data from six studies to investigate the determinants of the dietary acetate recovery factor (dARF) at rest and after physical activity interventions and compared the effects of different methods of dARF calculation on both the fat oxidation and its variability. In healthy lean subjects, dARF was 50.6 ± 5.4% dose ( n = 56) with an interindividual coefficient of variation of 10.6% at rest and 9.2% after physical activity modifications. The physical activity interventions did not impact dARF, and the intraindividual coefficient of variation was 4.6%. No major anthropological or physiological determinants were detected except for resting metabolic rate, which explains 7.4% of the dARF variability. Applying an individual or an average group dARF did not affect the mean and the variability of the derived dietary lipid oxidation at rest or after physical activity interventions. Using a mean dARF for a group leads to over- or underestimation of fat oxidation of less than 10% in individual subjects. Moreover, the use of a group or individual correction did not affect the significant relationship found between fasting respiratory exchange ratio and dietary fat oxidation. These data indicate that an average dARF can be applied for longitudinal and cross-sectional studies investigating dietary lipid metabolism.
- Subjects :
- Adult
Male
MESH: Oxidation-Reduction
medicine.medical_specialty
Physiology
Endocrinology, Diabetes and Metabolism
Rest
Physical Exertion
Palmitic Acid
stable isotopes
Acetates
MESH: Physical Exertion
Fat oxidation
Physiology (medical)
Internal medicine
TRACER
medicine
Humans
Dietary fat
mass spectrometry
MESH: Mass Spectrometry
Carbon Isotopes
Chromatography
MESH: Humans
MESH: Kinetics
Chemistry
[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]
MESH: Oleic Acid
MESH: Carbon Isotopes
exogenous fatty acid oxidation
MESH: Adult
Dietary Fats
MESH: Male
Kinetics
MESH: Breath Tests
Endocrinology
Biochemistry
Breath Tests
MESH: Dietary Fats
Female
MESH: Rest
MESH: Acetates
MESH: Palmitic Acid
Oxidation-Reduction
MESH: Female
Oleic Acid
Subjects
Details
- Language :
- English
- ISSN :
- 01931849 and 15221555
- Database :
- OpenAIRE
- Journal :
- AJP-Endocrinology and Metabolism, AJP-Endocrinology and Metabolism, American Physiological Society, 2008, 294 (4), pp.E645-E653. ⟨10.1152/ajpendo.00720.2007⟩
- Accession number :
- edsair.doi.dedup.....7eaa3d0e85b44b45d9107adc8bb27405