Coupling of chromatin structure to cell geometry during the cell cycle: transformed versus reverse-transformed CHO

The cellular and nuclear morphometry of synchronized CHO cells growing on glass in the presence or absence of 10 −3 M of cAMP has been determined. Measurements were made at various time intervals following selective mitotic detachment. The changes in nuclear morphometry during the cycle of cells growing on glass confirms previous findings on similar cells growing in suspension. When cells are grown on glass, the cell shape also changes. Specifically, when cells are treated with cAMP, the cytoplasmic cell shape and condensation is directly coupled with nuclear chromatin shape and condensation, but they are uncoupled for the transformed cells.