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Missense Mutations in the Human Insulin Promoter Factor-1 Gene and Their Relation to Maturity-Onset Diabetes of the Young and Late-Onset Type 2 Diabetes Mellitus in Caucasians*
- Source :
- The Journal of Clinical Endocrinology & Metabolism. 85:1323-1326
- Publication Year :
- 2000
- Publisher :
- The Endocrine Society, 2000.
-
Abstract
- Increasing evidence suggests that defects in genes encoding transcription factors that are expressed in the pancreatic β-cells may be important contributors to the genetic basis of type 2 diabetes mellitus. Maturity-onset diabetes of the young (MODY) now exists in five subtypes (MODY1–5), four of which are caused by mutations in transcription factors hepatocyte nuclear factor-4α (HNF-4α), HNF-1α, insulin promoter factor-1 (IPF-1), and HNF-1β (MODY1, -3, -4, and -5). Recent evidence from the British population even suggested that IPF-1 may be a predisposing gene for type 2 diabetes. Thus, highlighting the potential role of this transcription factor in the genetic basis of Danish and Italian MODY as well as in Danish patients with late-onset type 2 diabetes mellitus, we have examined the human IPF-1 gene for mutations by single strand conformation polymorphism and heteroduplex analysis in 200 Danish patients with late-onset type 2 diabetes and in 44 Danish and Italian MODY patients. In the patients with late-onset type 2 diabetes we identified a noncoding G insertion/deletion polymorphism at nucleotide −108, a silent G54G, and a rare missense D76N variant. Moreover, a Danish MODY patient was carrier of an A140T variant. Neither the D76N nor the A140T segregated with diabetes, and their transcriptional activation of the human insulin promoter expressed in vitro was indistinguishable from that of the wild type (115 ± 21% and 84 ± 12% vs. 100%). We conclude that variants in IPF-1 are not a common cause of MODY or late-onset type 2 diabetes in the Caucasian population, and that in terms of insulin transcription both the N76 and the T140 mutations are likely to represent functionally normal IPF-1 variants with no direct role in the pathogenesis of MODY or late-onset type 2 diabetes mellitus.
- Subjects :
- Adult
Male
Transcriptional Activation
Heterozygote
medicine.medical_specialty
Denmark
Endocrinology, Diabetes and Metabolism
medicine.medical_treatment
DNA Mutational Analysis
Clinical Biochemistry
Population
Mutation, Missense
Type 2 diabetes
Biology
Biochemistry
White People
Maturity onset diabetes of the young
Settore MED/13 - Endocrinologia
Mice
Endocrinology
Internal medicine
Diabetes mellitus
medicine
Animals
Humans
Age of Onset
education
Polymorphism, Single-Stranded Conformational
Aged
Aged, 80 and over
Homeodomain Proteins
Genetics
education.field_of_study
Insulin
Biochemistry (medical)
Type 2 Diabetes Mellitus
Promoter
3T3 Cells
DNA
Middle Aged
medicine.disease
Diabetes Mellitus, Type 2
Mutagenesis
Trans-Activators
Female
TCF7L2
Subjects
Details
- ISSN :
- 19457197 and 0021972X
- Volume :
- 85
- Database :
- OpenAIRE
- Journal :
- The Journal of Clinical Endocrinology & Metabolism
- Accession number :
- edsair.doi.dedup.....7f433525c932b896f0f6a082b248b829
- Full Text :
- https://doi.org/10.1210/jcem.85.3.6421