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Intracellular polarity protein PAR-1 regulates extracellular laminin assembly by regulating the dystroglycan complex
- Source :
- Genes to Cells. 14:835-850
- Publication Year :
- 2009
- Publisher :
- Wiley, 2009.
-
Abstract
- Cell polarity depends on extrinsic spatial cues and intrinsic polarity proteins including PAR-aPKC proteins. In mammalian epithelial cells, cell-cell contacts provide spatial cues that activate the aPKC-PAR-3-PAR-6 complex to establish the landmark of the initial cellular asymmetry. PAR-1, a downstream target of the aPKC-PAR-3-PAR-6 complex, mediates further development of the apical and basolateral membrane domains. However, the relationships between the PAR-aPKC proteins and other extrinsic spatial cues provided by the extracellular matrix (ECM) remain unclear. Here, we show that PAR-1 colocalizes with laminin receptors and is required for the assembly of extracellular laminin on the basal surface of epithelial cells. Furthermore, PAR-1 regulates the basolateral localization of the dystroglycan (DG) complex, one of the laminin receptors essential for basement membrane formation. We also show that PAR-1 interacts with the DG complex and is required for the formation of a functional DG complex. These results reveal the presence of a novel inside-out pathway in which an intracellular polarity protein regulates the ECM organization required for epithelial cell polarity and tissue morphogenesis.
- Subjects :
- Protein Serine-Threonine Kinases
Transfection
Receptors, Laminin
Extracellular matrix
Cell membrane
Dogs
Laminin
Cell polarity
Genetics
medicine
Extracellular
Dystroglycan
Animals
Dystroglycans
Cells, Cultured
Epithelial polarity
biology
Cell Membrane
Cell Polarity
Cell Biology
Extracellular Matrix
Dystroglycan complex
Cell biology
medicine.anatomical_structure
Microscopy, Fluorescence
biology.protein
Subjects
Details
- ISSN :
- 13652443 and 13569597
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Genes to Cells
- Accession number :
- edsair.doi.dedup.....7f46398971693d794aac1613db21a6ca