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Human papillomavirus 16 virus-like particles use heparan sulfates to bind dendritic cells and colocalize with langerin in Langerhans cells
- Source :
- The Journal of general virology. 86(Pt 5)
- Publication Year :
- 2005
-
Abstract
- Langerhans cells (LC), the immature dendritic cells (DC) that reside in epithelial tissues are among the first immune cells to encounter human papillomavirus (HPV) and are not activated by HPV virus-like particles (VLPs) in contrast to DC. The notion that the differences in response to HPV VLPs between LC and DC are associated with different types of cell binding and intracellular trafficking has been addressed. Inhibition experiments with heparin and sodium chlorate showed that heparan sulfates are necessary for HPV 16 VLPs to bind to DC but not to LC. Electron microscopy analysis demonstrated a colocalization of HPV 16 VLPs and langerin, which is expressed only by LC. This colocalization was observed on the cell surface but also in cytoplasmic vesicles. As anti-langerin antibodies, HPV 16 VLPs were associated with a faster entry kinetics in LC, as reflected by the fact that VLPs were observed near the nuclear membrane of LC within 10 min whereas more than 60 min were needed in DC. However, no difference between LC and DC was observed for the endocytosis pathway. HPV 16 VLPs entered in both DC and LC by a clathrin-dependent-pathway and were then localized in large cytoplasmic vesicles resembling endosomes.
- Subjects :
- Langerhans cell
Langerin
Virosomes
Endosome
viruses
Endocytosis Pathway
Cell membrane
Virus-like particle
Antigens, CD
Virology
medicine
Humans
Lectins, C-Type
Papillomaviridae
biology
Cell Membrane
Cytoplasmic Vesicles
virus diseases
Colocalization
Dendritic cell
Dendritic Cells
Cell biology
Microscopy, Electron
medicine.anatomical_structure
Mannose-Binding Lectins
Langerhans Cells
Antigens, Surface
biology.protein
Receptors, Virus
Heparitin Sulfate
Subjects
Details
- ISSN :
- 00221317
- Volume :
- 86
- Issue :
- Pt 5
- Database :
- OpenAIRE
- Journal :
- The Journal of general virology
- Accession number :
- edsair.doi.dedup.....7f5cf5ed77767bfc2f4c83e669fb55b8