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Innate Lymphoid Cells Groups 1 and 3 in the Epithelial Compartment of Functional Human Intestinal Allografts
- Source :
- American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 16(1)
- Publication Year :
- 2015
-
Abstract
- We examined intraepithelial lymphocytes (IELs) in 213 ileal biopsies from 16 bowel grafts and compared them with 32 biopsies from native intestines. During the first year posttransplantation, grafts exhibited low levels of IELs (percentage of CD103(+) cells) principally due to reduced CD3(+) CD8(+) cells, while CD103(+) CD3(-) cell numbers became significantly higher. Changes in IEL subsets did not correlate with histology results, isolated intestine, or multivisceral transplants, but CD3(-) IELs were significantly higher in patients receiving corticosteroids. Compared with controls, more CD3(-) IELs of the grafts expressed CD56, NKp44, interleukin (IL)-23 receptor, retinoid-related orphan receptor gamma t (RORγt), and CCR6. No difference was observed in granzyme B, and CD3(-) CD127(+) cells were more abundant in native intestines. Ex vivo, and after in vitro activation, CD3(-) IELs in grafts produced significantly more interferon (IFN)-γ and IL-22, and a double IFNγ(+) IL-22(+) population was observed. Epithelial cell-depleted grafts IELs were cytotoxic, whereas this was not observed in controls. In conclusion, different from native intestines, a CD3(-) IEL subset predominates in grafts, showing features of natural killer cells and intraepithelial ILC1 (CD56(+) , NKp44(+) , CCR6(+) , CD127(-) , cytotoxicity, and IFNγ secretion), ILC3 (CD56(+) , NKp44(+) , IL-23R(+) , CCR6(+) , RORγt(+) , and IL-22 secretion), and intermediate ILC1-ILC3 phenotypes (IFNγ(+) IL-22(+) ). Viability of intestinal grafts may depend on the balance among proinflammatory and homeostatic roles of ILC subsets.
- Subjects :
- 0301 basic medicine
Adult
Male
CD3 Complex
CD3
Population
chemical and pharmacologic phenomena
Biology
Lymphocyte Activation
digestive system
03 medical and health sciences
Young Adult
RAR-related orphan receptor gamma
Antigens, CD
T-Lymphocyte Subsets
Immunology and Allergy
Cytotoxic T cell
Humans
Pharmacology (medical)
education
Interleukin-7 receptor
Aged
Transplantation
education.field_of_study
hemic and immune systems
Epithelial Cells
Middle Aged
Allografts
Molecular biology
Granzyme B
Intestines
Killer Cells, Natural
Intestinal Diseases
030104 developmental biology
Case-Control Studies
Immunology
biology.protein
Intraepithelial lymphocyte
Cytokines
Female
Integrin alpha Chains
CD8
Subjects
Details
- ISSN :
- 16006143
- Volume :
- 16
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
- Accession number :
- edsair.doi.dedup.....7f7eaaa1c747e6988847f1f658d5ac0c