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Transcriptome analysis of genes associated with autolysis of Coprinus comatus

Transcriptome analysis of genes associated with autolysis of Coprinus comatus

Authors :
Hong-Bo, Guo
Zhi-Fei, Zhang
Jia-Qing, Wang
Si-Yu, Wang
Ji-Kang, Yang
Xi-Yao, Xing
Xiao-Jian, Qi
Xiao-Dan, Yu
Source :
Scientific Reports. 12
Publication Year :
2022
Publisher :
Springer Science and Business Media LLC, 2022.

Abstract

Coprinus comatus, widely known as “Jituigu”, is an important commodity and food in China. The yield of C. comatus, however, is substantially reduced by the autolysis of the fruiting bodies after harvest. To gain insight into the molecular mechanism underlying this autolysis, we divided the growth of C. comatus fruiting bodies into four stages: infant stage (I), mature stage (M), discolored stage (D), and autolysis stage (A). We then subjected these stages to de novo transcriptomic analysis using high-throughput Illumina sequencing. A total of 12,946 unigenes were annotated and analyzed with the Gene Ontology (GO), Clusters of Orthologous Groups of proteins (COG), and Kyoto Encyclopedia of Genes and Genomes (KEGG). We analyzed the differentially expressed genes (DEGs) between stages I and M, M and D, and D and A. Because the changes from M to D are thought to be related to autolysis, we focused on the DEGs between these two stages. We found that the pathways related to metabolic activity began to vary in the transition from M to D, including pathways named as autophagy—yeast, peroxisome, and starch and sucrose metabolism. This study also speculates the possible process of the autolysis of Coprinus comatus. In addition, 20 genes of interest were analyzed by quantitative real-time PCR to verify their expression profiles at the four developmental stages. This study, which is the first to describe the transcriptome of C. comatus, provides a foundation for future studies concerning the molecular basis of the autolysis of its fruiting bodies.

Details

ISSN :
20452322
Volume :
12
Database :
OpenAIRE
Journal :
Scientific Reports
Accession number :
edsair.doi.dedup.....7f99926c01c043929feea1934b9d7ac3
Full Text :
https://doi.org/10.1038/s41598-022-06103-z