Back to Search
Start Over
Evaluation on the performance of four different column models mounted on the compact type-I coil planet centrifuge
- Source :
- Journal of Chromatography A. 1217:7612-7615
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- Optimal positions of coiled separation columns on the type-I centrifuge were determined for four typical two-phase solvent systems to obtain the best separation efficiency (resolution and retention of stationary phase) for each with a suitable set of test samples. A set of short coiled columns is connected in series and mounted around the holder hub in four different ways: (model A) the tail of one unit with left-handedness was connected to the head of the next unit with right-handedness (TL-HR); (model B) the tail of one unit with left-handedness was connected to the tail of the next unit with right-handedness (TL-TR); (model C) the tail of one unit with left-handedness was connected to the tail of the next unit with left-handedness (TL-TL); (model D) the tail of one unit with left-handedness was connected to the head of the next unit with left-handedness (TL-HL). The results indicated that the performance of model D was the best among the four models. High revolution speed (800. rpm) is favorable to separation using the moderately hydrophobic solvent system of hexane-ethyl acetate-methanol-0.1. M HCl (1:1:1:1, v/v) (HEMW), while lower revolution speed (600. rpm) is beneficial to the separation with polar solvent system of 1-butanol-acetic acid-water (19:1:20, v/v) (BAW).
- Subjects :
- Centrifuge
Chromatography
Chemistry
Instrumentation
Organic Chemistry
Resolution (electron density)
Analytical chemistry
Centrifugation
General Medicine
Biochemistry
Chemistry Techniques, Analytical
Article
Analytical Chemistry
Countercurrent chromatography
Electromagnetic coil
Solvents
Head (vessel)
Polar
Hydrophobic and Hydrophilic Interactions
Unit (ring theory)
Subjects
Details
- ISSN :
- 00219673
- Volume :
- 1217
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography A
- Accession number :
- edsair.doi.dedup.....7fa4ef330ad3dcf8185d6214f9441add