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Sodium butyrate/retinoic acid costimulation induces apoptosis-independent growth arrest and cell differentiation in normal and ras-transformed seminal vesicle epithelial cells unresponsive to retinoic acid
- Source :
- Journal of molecular endocrinology. 24(1)
- Publication Year :
- 2000
-
Abstract
- Retinoic acid (RA) and sodium butyrate (NaB) are regulators of cell growth and differentiation. We studied their effect on normal (SVC1) or v-Ki-ras-transformed (Ki-SVC1) rat seminal vesicle (SV) epithelial cell lines. The treatment of these cells with 10(-((7( M RA did not produce significant changes in the morphological and biochemical parameters analyzed. When RA was used in combination with 2 mM NaB, the treatment induced substantial morphological changes, apoptosis-independent growth arrest, up-regulation of tissue transglutaminase (tTGase), and down-regulation of beta and gamma RA receptor (RAR) mRNA expression. The same cells did not express RAR alpha either before or after NaB/RA treatment. A similar treatment did not change the amount of mRNA coding for the protein SV-IV (a typical differentiation marker of the SV epithelium) in normal or ras-transformed cells nor the level of v-Ki-ras mRNA in Ki-SVC1 cells. These findings suggest that a defective RA/RARs signaling pathway is probably the biochemical condition that underlies the unresponsiveness to RA of our in vitro culture system, and indirectly points to the possibility that the NaB/RA-induced effects were brought about by a cooperation at the transcription level between the histone deacetylase inhibitory activity of NaB and the ability of RA/RAR to modulate the expression of various genes involved in the control of cell growth and differentiation.
- Subjects :
- Male
DNA Primer
Receptors, Retinoic Acid
Cellular differentiation
Retinoic acid
Down-Regulation
Apoptosis
Tretinoin
Biology
chemistry.chemical_compound
Endocrinology
Downregulation and upregulation
Seminal Vesicle
Animals
RNA, Messenger
Receptor
Molecular Biology
Cell Line, Transformed
DNA Primers
Epithelial Cell
Transglutaminases
Base Sequence
Cell growth
Animal
Apoptosi
Seminal Vesicles
Sodium butyrate
Butyrate
Cell Differentiation
Epithelial Cells
Genes, ra
Transglutaminase
Cell biology
Rats
Butyrates
Genes, ras
Biochemistry
chemistry
Rat
Signal transduction
Cell Division
Subjects
Details
- ISSN :
- 09525041
- Volume :
- 24
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of molecular endocrinology
- Accession number :
- edsair.doi.dedup.....7fa591792b15b30c64eeaa4678e8b1d0