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SIRT1, 2, 3 protect mouse oocytes from postovulatory aging
- Source :
- Aging (Albany NY)
- Publication Year :
- 2016
- Publisher :
- Impact Journals LLC, 2016.
-
Abstract
- The quality of metaphase II oocytes will undergo a time-dependent deterioration following ovulation as the result of the oocyte aging process. In this study, we determined that the expression of sirtuin family members (SIRT1, 2, 3) was dramatically reduced in mouse oocytes aged in vivo or in vitro. Increased intracellular ROS was observed when SIRT1, 2, 3 activity was inhibited. Increased frequency of spindle defects and disturbed distribution of mitochondria were also observed in MII oocytes aged in vitro after treatment with Nicotinamide (NAM), indicating that inhibition of SIRT1, 2, 3 may accelerate postovulatory oocyte aging. Interestingly, when MII oocytes were exposed to caffeine, the decline of SIRT1, 2, 3 mRNA levels was delayed and the aging-associated defective phenotypes could be improved. The results suggest that the SIRT1, 2, 3 pathway may play a potential protective role against postovulatory oocyte aging by controlling ROS generation.
- Subjects :
- 0301 basic medicine
Niacinamide
Aging
media_common.quotation_subject
nicotinamide
Spindle Apparatus
Biology
Mitochondrion
Andrology
Toxicology
03 medical and health sciences
Mice
0302 clinical medicine
Sirtuin 2
Sirtuin 1
In vivo
Sirtuin 3
medicine
Animals
Ovulation
Cellular Senescence
media_common
caffeine
postovulatory aging
Cell Biology
Oocyte
SIRT1, 2, 3
Spindle apparatus
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Sirtuin
biology.protein
Oocytes
Female
Reactive Oxygen Species
Intracellular
Research Paper
Subjects
Details
- Language :
- English
- ISSN :
- 19454589
- Volume :
- 8
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Aging (Albany NY)
- Accession number :
- edsair.doi.dedup.....7fa8e8dadedba2442b6d727f5fd1506c