Back to Search
Start Over
An improved LC-MS/MS method for quantitative determination of ilaprazole and its metabolites in human plasma and its application to a pharmacokinetic study
- Source :
- Acta pharmacologica Sinica. 30(9)
- Publication Year :
- 2009
-
Abstract
- To improve and validate an analytical method based on liquid chromatography and electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) for the quantitative measurement of ilaprazole and its two metablites in human plasma.Separation of analytes and the internal standard (IS), omeprazole, was performed on a Thermo HyPURITY C18 column (150x2.1 mm, 5 microm) with a mobile phase consisting of 10 mmol/L ammonium formate water-acetonitrile solution (50:50, v/v) at a flow rate of 0.25 mL/min. The API4000 triple quadruple mass spectrometer was operated in multiple reactions monitoring mode via positive electrospray ionization interface using the transition m/z 367.2 --m/z184.0 for ilaprazole, m/z 383.3 --m/z 184.1 for ilaprazole sulfone, m/z 351.2 --m/z 168.1 for ilaprazole thiol ether and m/z 346.2 --m/z 198.0 for omeprazole.The method was linear over the concentration range of 0.23-2400.00 ng/mL for ilaprazole, 0.05-105.00 ng/mL for ilaprazole thiol ether and 0.06-45.00 ng/mL for ilaprazole sulfone. The intra- and inter-day precisions were all less than 15% in terms of relative standard deviation (RSD), and the accuracy was within 15% in terms of relative error (RE) for ilaprazole, ilaprazole sulfone and ilaprazole thiol ether. The lower limit of quantification (LLOQ) was identifiable and reproducible at 0.23, 0.05 and 0.06 ng/mL with acceptable precision and accuracy for ilaprazole, ilaprazole sulfone and ilaprazole thiol ether, respectively.The validated method offered sensitivity and a wide linear concentration range. This method was successfully applied for the evaluation of the pharmacokinetics of ilaprazole and its two metabolites after single oral doses of 5 mg ilaprazole to 12 healthy Chinese volunteers.
- Subjects :
- Pharmacology
Adult
Male
Spectrometry, Mass, Electrospray Ionization
Chromatography
Ilaprazole
Metabolite
Chromatography liquid
General Medicine
Quantitative determination
2-Pyridinylmethylsulfinylbenzimidazoles
chemistry.chemical_compound
Young Adult
chemistry
Pharmacokinetics
Human plasma
Sulfoxides
Lc ms ms
Humans
Pharmacology (medical)
Benzimidazoles
Original Article
Sulfones
Ilaprazole sulfone
Chromatography, Liquid
Subjects
Details
- ISSN :
- 17457254
- Volume :
- 30
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Acta pharmacologica Sinica
- Accession number :
- edsair.doi.dedup.....800ce7ea4c19c1141bd9adb79e4da649