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A novel vacuolar membrane H+-ATPase c subunit gene (ThVHAc1) from Tamarix hispida confers tolerance to several abiotic stresses in Saccharomyces cerevisiae
- Source :
- Molecular biology reports. 38(2)
- Publication Year :
- 2009
-
Abstract
- Plant vacuolar H(+)-ATPase (V-ATPase) plays an important role in response to different adverse environmental conditions. In the present study, we cloned and characterized a V-ATPase c subunit gene (ThVHAc1) from Tamarix hispida. The deduced ThVHAc1 amino acid sequence lacks a signal peptide and ThVHAc1 is a highly hydrophobic protein with four transmembrane regions. A transient expression assay showed that the ThVHAc1-GFP fusion protein is expressed on onion epidermal endomembrane cells. Real-time RT-PCR demonstrated that ThVHAc1 gene expression was induced by NaCl, NaHCO(3), PEG and CdCl(2) stress in T. hispida roots, stems and leaves. Exogenous application of abscisic acid (ABA) also stimulated ThVHAc1 transcript levels in the absence of stress, suggesting that ThVHAc1 is involved in ABA-dependent stress signaling pathway. Furthermore, the transgenic yeast expressing ThVHAc1 increased salt, drought, ultraviolet (UV), oxidative, heavy metal, cold and high temperature tolerance. Our results suggested that the ThVHAc1 gene from T. hispida serves a stress tolerance role in the species.
- Subjects :
- Signal peptide
Vacuolar Proton-Translocating ATPases
DNA, Complementary
Time Factors
ATPase
Saccharomyces cerevisiae
Green Fluorescent Proteins
Genes, Plant
chemistry.chemical_compound
Gene expression
Onions
Genetics
Transgenes
Molecular Biology
Abscisic acid
Peptide sequence
Plant Diseases
Regulation of gene expression
biology
Reverse Transcriptase Polymerase Chain Reaction
Tamaricaceae
Gene Expression Profiling
General Medicine
biology.organism_classification
Tamarix hispida
chemistry
Biochemistry
Gene Expression Regulation
biology.protein
Abscisic Acid
Subjects
Details
- ISSN :
- 15734978
- Volume :
- 38
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Molecular biology reports
- Accession number :
- edsair.doi.dedup.....801acb5f9778bf9da18da2fa926154ad