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Spatial mapping of integrin interactions and dynamics during cell migration by Image Correlation Microscopy
- Source :
- Journal of Cell Science. 117:5521-5534
- Publication Year :
- 2004
- Publisher :
- The Company of Biologists, 2004.
-
Abstract
- Image correlation microscopy methodology was extended and used to determine retrospectively the density, dynamics and interactions of alpha5-integrin in migrating cells. Alpha5-integrin is present in submicroscopic clusters containing 3-4 integrins before it is discernibly organized. The integrin in nascent adhesions, as identified by the presence of paxillin, is approximately 1.4 times more concentrated, approximately 4.5 times more clustered and much less mobile than in surrounding regions. Thus, while integrins are clustered throughout the cell, they differ in nascent adhesions and appear to initiate adhesion formation, despite their lack of visible organization. In more mature adhesions where the integrin is visibly organized there are approximately 900 integrins microm(-2) (about fivefold higher than surrounding regions). Interestingly, alpha5-integrin and alpha-actinin, but not paxillin, reside in a complex throughout the cell, where they diffuse and flow together, even in regions where they are not organized. During adhesion disassembly some integrins diffuse away slowly, alpha-actinin undergoes a directed movement at speeds similar to actin retrograde flow (0.29 microm min(-1)), while all of the paxillin diffuses away rapidly.
- Subjects :
- Integrins
Integrin
Cell
Cytoplasmic Streaming
CHO Cells
Integrin alpha5
Diffusion
Mice
Cell Movement
Cricetinae
Microscopy
Cell Adhesion
Image Processing, Computer-Assisted
medicine
Animals
Actinin
Cytoskeleton
Actin
Paxillin
Image Cytometry
Microscopy, Confocal
biology
Cell Membrane
Dynamics (mechanics)
Cell migration
Cell Biology
Adhesion
Models, Theoretical
Phosphoproteins
Cell biology
Cytoskeletal Proteins
Protein Transport
medicine.anatomical_structure
biology.protein
Algorithms
Subjects
Details
- ISSN :
- 14779137 and 00219533
- Volume :
- 117
- Database :
- OpenAIRE
- Journal :
- Journal of Cell Science
- Accession number :
- edsair.doi.dedup.....8035611da4cffca7c2828d3af8f4614d