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Author Correction: Exfoliative toxin E, a new Staphylococcus aureus virulence factor with host-specific activity
- Source :
- Scientific Reports, Vol 10, Iss 1, Pp 1-2 (2020), Scientific Reports, Scientific Reports, Nature Publishing Group, 2020, 10 (1), ⟨10.1038/s41598-020-59360-1⟩, file:///C:/Users/agiboulot/Desktop/s41598-020-59360-1.pdf, Scientific Reports, . (2019)
- Publication Year :
- 2020
- Publisher :
- Nature Publishing Group, 2020.
-
Abstract
- Exfoliative toxins (ETs) are secreted virulence factors produced by staphylococci. These serine proteases specifically cleave desmoglein 1 (Dsg1) in mammals and are key elements in staphylococcal skin infections. We recently identified a new et gene in S. aureus O46, a strain isolated from ovine mastitis. In the present study, we characterized the new et gene at a genetic level and the enzymatic activity of the deduced protein. The S. aureus O46 genome was re-assembled, annotated and compared with other publicly available S. aureus genomes. The deduced amino acid sequence of the new et gene shared 40%, 53% and 59% sequence identity to those of ETA, ETB and ETD, respectively. The new et gene shared the same genetic vicinity and was similar in other S. aureus strains bearing this gene. The recombinant enzyme of the new et gene caused skin exfoliation in vivo in neonatal mice. The new et-gene was thus named ete, encoding a new type (type E) of exfoliative toxin. We showed that ETE degraded the extracellular segments of Dsg1 in murine, ovine and caprine epidermis, as well as in ovine teat canal epithelia, but not that in bovine epidermis. We further showed that it directly hydrolyzed human and swine Dsg1 as well as murine Dsg1α and Dsg1β, but not canine Dsg1 or murine Dsg1γ. Molecular modeling revealed a correlation between the preferred orientation of ETE docking on its Dsg1 cleavage site and species-specific cleavage activity, suggesting that the docking step preceding cleavage accounts for the ETE species-specificity. This new virulence factor may contribute to the bacterial colonization on the stratified epithelia in certain ruminants with mastitis.
- Subjects :
- Exfoliative toxin
Staphylococcus aureus
staphylococcus areus
Protein Conformation
exfoliative toxin
lcsh:Medicine
approche génomique
Biology
medicine.disease_cause
serine protéase
Host Specificity
Virulence factor
toxine exfoliative
Microbiology
Mice
03 medical and health sciences
mammite
staphylococcus aureus
virulence
protéase
serine
génomique
0302 clinical medicine
medicine
Animals
Amino Acid Sequence
Author Correction
lcsh:Science
Host specific
Toxins, Biological
030304 developmental biology
0303 health sciences
Multidisciplinary
santé animale
Hydrolysis
Microbiology and Parasitology
lcsh:R
Ruminants
Microbiologie et Parasitologie
3. Good health
Molecular Docking Simulation
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
lcsh:Q
Extracellular Space
Genome, Bacterial
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....8045f761538726818919ad3a18e52201