Back to Search
Start Over
Melatonin and calcium function synergistically to promote the resilience through ROS metabolism under arsenic-induced stress
- Source :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Publication Year :
- 2020
-
Abstract
- The interplay between melatonin (Mel) and calcium (Ca) in enhancing tolerance to metalloid toxicity and underlying physiological and biochemical mechanisms of this relationship still remains unknown. The present study reveals that the signaling molecules Mel and/or Ca enhanced tolerance of Vicia faba (cv. Tara) plant to metalloid arsenic (As) toxicity. However, a combination of Mel and Ca was more efficient than alone. Plants grew with As exhibited enhanced hydrogen peroxide, superoxide anion, electrolyte leakage, lipid peroxidation together with increased reactive oxygen species (ROS) producing enzymes, such as NADPH oxidase and glycolate oxidase (GOX). On the contrary, an inhibition in chlorophyll (Chl) biosynthesis and gas exchange parameters (net photosynthetic rate, stomatal conductance, intercellular carbon dioxide concentration) was observed. Under As toxicity conditions, the application of Mel and Ca synergistically suppressed the plants’ program cell death features (nucleus condensation and nucleus fragmentation) in guard cells of stomata, DNA damage, and formation of ROS in guard cells, leaves and roots. Moreover, it enhanced gas exchange parameters and activity of enzymes involved in photosynthesis process (carbonic anhydrase and RuBisco), Chl biosynthesis (δ-aminolevulinic acid dehydratase), and decreased activity of Chl degrading enzyme (chlorophyllase) under As toxicity conditions. Our investigation evidently established that expression of ATP synthase, Ca-ATPase, Ca-DPKase, Hsp17.6 and Hsp40 was found maximum in the plants treated with Mel + Ca, resulting in higher tolerance of plants to As stress. Also, increased total soluble carbohydrates, cysteine, and Pro accumulation with increased Pro synthesizing enzyme (Δ-pyrroline-5-carboxylate synthetase (P5CS) and decreased Pro degrading enzyme (proline dehydrogenase) in Mel + Ca treated plants conferred As toxicity tolerance. The obtained results postulate strong evidence that the application of Mel along with Ca enhances resilience against As toxicity by upregulating the activity of plasma membrane H-ATPase, enzymes involved in antioxidant system, and ascorbate-glutathione pathway.<br />The authors extend their appreciation to the Deanship of Scientific Research at King Saud University for funding this work through Research Group No. RG-1441-438.
- Subjects :
- Chlorophyll
Environmental Engineering
Antioxidant
Health, Toxicology and Mutagenesis
medicine.medical_treatment
Metalloid toxicity
0211 other engineering and technologies
Apoptosis
02 engineering and technology
010501 environmental sciences
01 natural sciences
Antioxidants
Arsenic
chemistry.chemical_compound
Guard cell
medicine
Environmental Chemistry
Photosynthesis
Waste Management and Disposal
Stomata
0105 earth and related environmental sciences
Melatonin
chemistry.chemical_classification
021110 strategic, defence & security studies
Reactive oxygen species
NADPH oxidase
biology
Superoxide
RuBisCO
food and beverages
Metabolism
Pollution
Chlorophyll degradation
Plant Leaves
Enzyme
chemistry
Biochemistry
Seedlings
biology.protein
Calcium
Proline metabolism
Reactive Oxygen Species
Subjects
Details
- ISSN :
- 18733336
- Volume :
- 398
- Database :
- OpenAIRE
- Journal :
- Journal of hazardous materials
- Accession number :
- edsair.doi.dedup.....804f4e8dfbc1c673f35bec21545dc3ed