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5-Azacytidine is able to induce the conversion of teratocarcinoma-derived mesenchymal cells into epithelia cells
- Source :
- The EMBO Journal. 3:961-967
- Publication Year :
- 1984
- Publisher :
- Wiley, 1984.
-
Abstract
- The inhibitor of DNA-methylation, 5-azacytidine (5- AzaC ) induced the appearance of cytokeratin-containing cells in several mesenchymal cell lines such as teratocarcinoma-derived fibroblasts, preadipocytes and myoblasts, NIH-3T3 fibroblasts and human embryonic fibroblasts. At optimal 5- AzaC concentrations the proportion of such cells was in the range of 10(-1) compared with 10(-6) -10(-4) in non-treated cultures. Dose-response curves indicated that the induction of cytokeratin was the result of an interaction of the drug with few targets. Stable, mature, keratinocyte cell lines, as well as lines of myoblasts and astrocytes, could be isolated from a teratocarcinoma-derived preadipocyte line, showing that 5- AzaC is able to provoke a wide range of complete phenotypic conversions. In these cell lines, the intermediate filaments corresponded to the morphological phenotype. Altogether, the results suggest that 5- AzaC preferentially activates certain genes.
- Subjects :
- Cellular differentiation
Mesenchyme
Fluorescent Antibody Technique
Biology
Epithelium
General Biochemistry, Genetics and Molecular Biology
Cell Line
Mice
Keratin
medicine
Animals
Humans
Fibroblast
Molecular Biology
Cells, Cultured
chemistry.chemical_classification
General Immunology and Microbiology
General Neuroscience
Mesenchymal stem cell
Teratoma
Cell Differentiation
Embryonic stem cell
Clone Cells
Cell biology
Kinetics
Phenotype
medicine.anatomical_structure
Adipose Tissue
chemistry
Cell culture
Immunology
Azacitidine
Keratinocyte
Research Article
Subjects
Details
- ISSN :
- 02614189
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- The EMBO Journal
- Accession number :
- edsair.doi.dedup.....809fad86b346fe4d7fb95f970d843a65
- Full Text :
- https://doi.org/10.1002/j.1460-2075.1984.tb01914.x