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Role of the alpha v-integrin subunit in cell proliferation, apoptosis and tumor metastasis of laryngeal and hypopharyngeal squamous cell carcinomas: a clinical and in vitro investigation
- Source :
- European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery. 266(1)
- Publication Year :
- 2007
-
Abstract
- The objective of this study was to investigate the role of alpha v-integrin subunit (ITGAV, CD51) in invasion and metastasis of the laryngeal and hypopharyngeal squamous cell carcinomas and to evaluate whether an antisense oligonucleotide sequence (ASONs) targeting ITGAV gene can result in proliferative inhibition and induce to apoptosis of laryngeal carcinoma cell lines (Hep-2). Firstly, a tissue microarray contained 75 primary carcinomas, 29 non-cancerous normal tissues and 20 metastatic lymph nodes was constructed and used to detect the expression of ITGAV by immunohistochemistry. The changes of ITGAV expression from each group were assessed and correlated to the clinical parameters of the patients. Secondly, the ASONs targeting ITGAV gene was transfected into Hep-2 cells in vitro. The proliferative ability of the cells after transfection was measured by MTT assay and the apoptosis was detected using flow cytometry. Results showed that the expression of ITGAV was significantly correlated with differentiation and lymph node metastasis of these cancers. In vitro test showed that the proliferative ability of Hep-2 cells was significantly inhibited by ASONs in a way of concentration- and time-depending mode, and a significant apoptosis of Hep-2 cells was also observed after ASONs transfection. In conclusion, the expression of ITGAV was significantly correlated with differentiation and metastasis of the laryngeal and hypopharyngeal carcinomas; down-regulation of ITGAV gene could inhibit proliferation of Hep-2 cells and induce to its apoptosis. These results suggest that ITGAV gene may become a promising prognostic marker and new treatment target for these cancers.
- Subjects :
- Adult
Male
Pathology
medicine.medical_specialty
Cell
Apoptosis
Biology
Sensitivity and Specificity
Flow cytometry
Metastasis
Tissue Culture Techniques
Cell Line, Tumor
medicine
Biomarkers, Tumor
Humans
Neoplasm Invasiveness
RNA, Messenger
ITGAV
Laryngeal Neoplasms
Aged
Cell Proliferation
Probability
Aged, 80 and over
Analysis of Variance
Tissue microarray
Hypopharyngeal Neoplasms
medicine.diagnostic_test
Reverse Transcriptase Polymerase Chain Reaction
General Medicine
Transfection
Middle Aged
medicine.disease
Flow Cytometry
Integrin alphaVbeta3
Gene Expression Regulation, Neoplastic
medicine.anatomical_structure
Otorhinolaryngology
Case-Control Studies
Cancer research
Carcinoma, Squamous Cell
Immunohistochemistry
Female
Subjects
Details
- ISSN :
- 14344726
- Volume :
- 266
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
- Accession number :
- edsair.doi.dedup.....80b48637c954c8099bddcbaa7d960074