Identification of functional epitopes of structural proteins and in-silico designing of dual acting multiepitope anti-tick vaccine against emerging Crimean-Congo hemorrhagic fever virus

Recurrent outbreaks of Crimean-Congo hemorrhagic fever (CCHF) virus infection in different parts of world are a major global health concern. The CCHF viral infection is associated with severe hemorrhagic fevers and mortality up to 40%. More than 30 countries in Asia, Europe and Africa are affected with CCHF infection. Prevention of infection through vaccine becomes more important when no effective antiviral and associated therapies are available. Further ticks play a crucial role in maintenance and transmission of CCHFV. Therefore, the control of transmission by ticks is warranted for ultimate prevention of outbreak. The study employed a series of immunoinformatics approaches to design novel multiepitope vaccine targeting highly immunodominant epitopes of major structural proteins (Nucleoprotein and Glycoprotein complex) of CCHFV. Vaccine was designed by incorporating linear and conformational B cell, helper and cytotoxic T cell epitopes from these crucial immunogenic proteins adjoined with appropriate linkers and adjuvant. This vaccine construct was also complemented with a highly immunogenic and conserved protective tick salivary antigen named subolesin to impart dual activity as a unique transmission blocking vaccine. The B-cell peptides were also experimentally validated. The designed vaccine was further in silico validated for its physiochemical properties, allergenicity and immunogenicity etc. The proposed candidate vaccine construct has the potential to function both as a vaccine against CCHF virus as well as a universal anti-tick vaccine.