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Characterization of the promoter of the mouse prohormone convertase PC2 gene
- Source :
- Endocrine journal. 45(4)
- Publication Year :
- 1999
-
Abstract
- Prohormone convertase 2 (PC2) is a member of a family of mammalian subtilisin-like endoproteases that are involved in the processing of prohormones, neuropeptides and many other precursor derived proteins. The expression of PC2 is restricted to neuroendocrine tissues such as pancreatic islets, the pituitary and the brain. To understand the regulation of the PC2 gene, we cloned and characterized the promoter region of the mouse PC2 gene. The transcriptional start site of the mouse PC2 gene is identical to that of the human. There is 79% identity in the sequences of the promoter regions between the mouse and human PC2 genes. The mouse PC2 gene, like the human, does not have a TATA-like motif in the region just upstream of the start of the transcription. Studies with promoter-reporter gene, chloramphenicol acetyltransferase (CAT), constructs showed that the region from -400 to -170 bp was necessary for high level expression of the mouse PC2 gene in the betaTC-3 insulinoma cells.
- Subjects :
- endocrine system
Transcription, Genetic
Endocrinology, Diabetes and Metabolism
Molecular Sequence Data
Restriction Mapping
Prohormone convertase
Proprotein convertase 2
Biology
Chloramphenicol acetyltransferase
Mice
Endocrinology
Restriction map
Transcription (biology)
medicine
Animals
Humans
Subtilisins
Promoter Regions, Genetic
Gene
Base Sequence
Pancreatic islets
Promoter
Molecular biology
Neoplasm Proteins
medicine.anatomical_structure
Proprotein Convertase 2
Subjects
Details
- ISSN :
- 09188959
- Volume :
- 45
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Endocrine journal
- Accession number :
- edsair.doi.dedup.....80dd6a88583f37315cb353f830430855