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Effectiveness and toxicity of a novel isolated actinomycete strain Streptomyces sp. JS01 on a harmful alga Phaeocystis globosa
- Source :
- Applied Microbiology and Biotechnology, Applied Microbiology and Biotechnology, Springer Verlag, 2015, 99 (11), pp.4807-14. ⟨10.1007/s00253-014-6349-4⟩
- Publication Year :
- 2014
-
Abstract
- An aquatic actinomycete capable of eliminating the brown tide causing marine alga Phaeocystis globosa was isolated from the surface sea water and the isolate named JS01 was characterized as Streptomyces on the basis of its 16S rRNA gene sequence. The supernatant of JS01 could lyse algal cells, implying that JS01 produced a latent alga-lytic compound. Considering this algicidal activity and the response of the algal cells, Chlorophyll a fluorescence decreased significantly in P. globosa in response to the JS01 supernatant when analyzed with flow cytometry. The algal cells experienced cell shrinkage and plasmolysis before disintegration after 72 h of treatment. The released algicide(s) were heat-tolerant, except above 121 °C, and fluctuation in pH variations; even so, algicidal activity was also over 60 %. The maximum toxicity of JS01 was on the seventh day of culture, and the relative luminosity was 0.49 at that time when detected by luminous bacteria Vibrio fischeri. These results indicated that the Streptomyces sp. JS01 could function as a potential controller of Phaeocystis globosa blooms.
- Subjects :
- 0106 biological sciences
Chlorophyll
DNA, Bacterial
Lysis
Cell Survival
Molecular Sequence Data
01 natural sciences
Applied Microbiology and Biotechnology
Streptomyces
Algal bloom
DNA, Ribosomal
Plasmolysis
Microbiology
03 medical and health sciences
Anti-Infective Agents
RNA, Ribosomal, 16S
Botany
Antibiosis
Cluster Analysis
14. Life underwater
ComputingMilieux_MISCELLANEOUS
Phylogeny
030304 developmental biology
[SDU.OCEAN]Sciences of the Universe [physics]/Ocean, Atmosphere
0303 health sciences
biology
010604 marine biology & hydrobiology
Chlorophyll A
Haptophyta
General Medicine
Sequence Analysis, DNA
biology.organism_classification
16S ribosomal RNA
Flow Cytometry
6. Clean water
Vibrio
Bacteria
Biotechnology
Subjects
Details
- ISSN :
- 14320614 and 01757598
- Volume :
- 99
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Applied microbiology and biotechnology
- Accession number :
- edsair.doi.dedup.....80ddf4cf612553528b2440afec46e130