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BST-2 restricts IAV release and is countered by the viral M2 protein
- Source :
- The Biochemical journal. 474(5)
- Publication Year :
- 2016
-
Abstract
- BST-2 (tetherin, CD317, and HM1.24) is induced by interferon and restricts virus release by tethering the enveloped viruses to the cell surface. The effect of BST-2 on influenza A virus (IAV) infection has been inconclusive. In the present study, we report that BST-2 diminishes the production of IAV virus-like particles (VLPs) that are generated by viral neuraminidase and hemagglutinin proteins to a much greater degree than it inhibits the production of wild-type IAV particles. This relatively weaker inhibition of IAV is associated with reduction in BST-2 levels, which is caused by the M2 protein that interacts with BST-2 and leads to down-regulation of cell surface BST-2 via the proteasomal pathway. Similarly to the viral antagonist Vpu, M2 also rescues the production of human immunodeficiency virus-1 VLPs and IAV VLPs in the presence of BST-2. Replication of wild-type and the M2-deleted viruses were both inhibited by BST-2, with the M2-deleted IAV being more restricted. These data reveal one mechanism that IAV employs to counter restriction by BST-2.
- Subjects :
- 0301 basic medicine
Proteasome Endopeptidase Complex
viruses
Hemagglutinin (influenza)
Neuraminidase
Hemagglutinin Glycoproteins, Influenza Virus
Biology
medicine.disease_cause
GPI-Linked Proteins
Transfection
Biochemistry
Madin Darby Canine Kidney Cells
Viral Matrix Proteins
03 medical and health sciences
Dogs
Influenza A Virus, H1N1 Subtype
Viral envelope
Interferon
Antigens, CD
Chlorocebus aethiops
Influenza A virus
medicine
Viral neuraminidase
Animals
Humans
Molecular Biology
Vero Cells
Virus Release
Innate immune system
Virion
Cell Biology
Virology
Recombinant Proteins
030104 developmental biology
HEK293 Cells
Gene Expression Regulation
Host-Pathogen Interactions
biology.protein
Tetherin
HIV-1
medicine.drug
HeLa Cells
Plasmids
Signal Transduction
Subjects
Details
- ISSN :
- 14708728
- Volume :
- 474
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- The Biochemical journal
- Accession number :
- edsair.doi.dedup.....810691188c52ed15f376ec062cad707d