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Gel-free electrophoresis of DNA and proteins on chips featuring a 70 nm capillary-well motif
- Source :
- ACS nano. 9(1)
- Publication Year :
- 2014
-
Abstract
- We present an integrated glass capillary system on silicon for size-based sieving of distinct mixtures of proteins, short DNA, and long DNA fragments into sharp peaks. The minimum resolvable size difference achieved is noted as 3.45 kDa for 45-52.8 kDa proteins, 20 bp for 200-300 bp DNA strands, and 182 bp for 5.6-5.8 kbp DNA chains. This high-resolution sieving arises from vastly steep entropic barriers created at the onsets of extremely restrictive (resistive) capillary segments and their pivotal role in shifting the equilibrium entropic sieving to intense fields (1000 V/cm). DNA fragments of various sizes are shown fully resolved in less than 7 min at a steady voltage of 2000 V being directly applied across the length of a 2 cm long sieve featuring thousands of entropic barriers. The utility of higher field strengths and longer sieves is also demonstrated without triggering dielectric breakdown by time-division multiplexing up to 2000 V across the 1 cm long sieve segments. The self-enclosed 70 nm diameter capillaries were fabricated using coarse (1 μm) photolithography and standard semiconductor manufacturing techniques.
- Subjects :
- Gel free
Silicon
Chemistry
Capillary action
General Engineering
Analytical chemistry
General Physics and Astronomy
chemistry.chemical_element
Proteins
Nanofluidics
DNA
law.invention
Electrophoresis, Microchip
Molecular Weight
Electrophoresis
Sieve
chemistry.chemical_compound
law
Humans
General Materials Science
Glass
Size difference
Subjects
Details
- ISSN :
- 1936086X
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- ACS nano
- Accession number :
- edsair.doi.dedup.....816863ecb22b28a3aa4175a3427b8311