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Signaling lymphocytic activation molecule (SLAM) is differentially expressed in human Th1 and Th2 cells

Authors :
Susanne Meissner
Riitta Lahesmaa
Heli Hamalainen
Source :
Journal of Immunological Methods. 242:9-19
Publication Year :
2000
Publisher :
Elsevier BV, 2000.

Abstract

We have used a real-time quantitative RT-PCR technique (TaqMan, PE Biosystems) to identify genes that are differentially expressed by human polarised CD4(+) T cell subsets (Th1 or Th2). The goal was to test the feasibility of the detection method in profiling the expression of a set of marker genes important for Th1 and Th2 differentiation. We demonstrate that in polarised human Th1 cells signaling lymphocytic activation molecule (SLAM), a member of the immunoglobulin superfamily, is expressed at 7-25-fold higher levels than in Th2 cells. Along with SLAM, expression of the IL-12 receptor chain beta 2 (IL-12R beta 2) and the IFN-gamma receptor chain beta (IFN-gamma R beta) proved to be useful molecular markers indicating the state of T cell polarisation, as previously reported. Treatment with IL-12 increased SLAM mRNA expression in T cells by 3-4-fold, whereas a number of other cytokines including PDGF-BB, IFN-alpha A, IFN-alpha A/D, IFN-beta, IFN-gamma or IL-9 had no effect. Stimulating T cells by co-ligating CD3 and CD28 increased SLAM protein surface expression in both Th1 and Th2 cells. In conclusion, real-time RT-PCR detection was found to be an accurate, sensitive and highly reproducible method for fast profiling of mRNA expression in Th1 and Th2 cell subsets.

Details

ISSN :
00221759
Volume :
242
Database :
OpenAIRE
Journal :
Journal of Immunological Methods
Accession number :
edsair.doi.dedup.....8173f342940490dd6b9938073971aa91
Full Text :
https://doi.org/10.1016/s0022-1759(00)00200-3