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Highly selective detection of spermine in human urine via a nanometal surface energy transfer platform
- Source :
- Talanta. 188:218-224
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- As an important biomarker of malignant tumors, spermine is closely related with some diseases. In this work, a nanometal surface energy transfer (NSET) strategy via the positively charged gold nanorods (AuNRs) and the negatively charged tetrakis (4-sulfonatophenyl) porphyrin (TPPS4) was developed to detect spermine in human urine. Under acidic condition, spermine possessed multi-cationic property and a strong affinity towards the anionic phosphate backbone of calf thymus DNA (ctDNA) by electrostatic attraction as well as the groove binding, which enabled to regulate the process of NSET between AuNRs and TPPS4, leading to the fluorescence quenching of TPPS4. Moreover, the quenched fluorescence was proportional to the concentration of spermine, which was applicable to monitor the level of spermine in human urine in the concentration range of 0.5–7.5 μM. The NSET platform for spemine is simple, selective and time-saving, which has great significance in early cancer diagnosis.
- Subjects :
- Porphyrins
Spermine
02 engineering and technology
Urine
01 natural sciences
Fluorescence
Analytical Chemistry
chemistry.chemical_compound
Limit of Detection
Animals
Humans
Nanotubes
Chemistry
010401 analytical chemistry
DNA
021001 nanoscience & nanotechnology
Phosphate
Porphyrin
0104 chemical sciences
Spectrometry, Fluorescence
Energy Transfer
Models, Chemical
Biophysics
Cattle
Nanorod
Gold
0210 nano-technology
Surface energy transfer
Subjects
Details
- ISSN :
- 00399140
- Volume :
- 188
- Database :
- OpenAIRE
- Journal :
- Talanta
- Accession number :
- edsair.doi.dedup.....817dd81abec179c42e8fc4e667e1d7e2
- Full Text :
- https://doi.org/10.1016/j.talanta.2018.05.086