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Highly selective detection of spermine in human urine via a nanometal surface energy transfer platform

Authors :
Hong Zhi Zhang
Ni Wang
Dan Yuan
Hong Yan Zou
Jia Jun Liu
Cheng Zhi Huang
Jian Wang
Source :
Talanta. 188:218-224
Publication Year :
2018
Publisher :
Elsevier BV, 2018.

Abstract

As an important biomarker of malignant tumors, spermine is closely related with some diseases. In this work, a nanometal surface energy transfer (NSET) strategy via the positively charged gold nanorods (AuNRs) and the negatively charged tetrakis (4-sulfonatophenyl) porphyrin (TPPS4) was developed to detect spermine in human urine. Under acidic condition, spermine possessed multi-cationic property and a strong affinity towards the anionic phosphate backbone of calf thymus DNA (ctDNA) by electrostatic attraction as well as the groove binding, which enabled to regulate the process of NSET between AuNRs and TPPS4, leading to the fluorescence quenching of TPPS4. Moreover, the quenched fluorescence was proportional to the concentration of spermine, which was applicable to monitor the level of spermine in human urine in the concentration range of 0.5–7.5 μM. The NSET platform for spemine is simple, selective and time-saving, which has great significance in early cancer diagnosis.

Details

ISSN :
00399140
Volume :
188
Database :
OpenAIRE
Journal :
Talanta
Accession number :
edsair.doi.dedup.....817dd81abec179c42e8fc4e667e1d7e2
Full Text :
https://doi.org/10.1016/j.talanta.2018.05.086