Supplemental Methods, Figures 1-2, Table S1 from Standardized Ki67 Diagnostics Using Automated Scoring—Clinical Validation in the GeparTrio Breast Cancer Study

Supplemental Methods, Figures 1-2, Table S1. Supplemental Methods providing details on the study cohort and the image analysis algorithm. Supp. Figure 1: A) Ki67 stained breast cancer tissue. B) Haematoxylin Signal derived from color deconvolution (1) and C) histogram showing frequencies (y axis) of specific heamatoxylin intensities (x axis) of the image background (red) and foreground (green). Arrows show representative locations of different intensity classes: no tissue (red), tissue but no cell nuclei (blue), Ki67 negative cell (green) and Ki67 positive cell nuclei (orange). Figure 2: Construction of the borderline between plausible and non-plausible thresholds by combining the threshold and the resulting ratio of positive cells. Supplementary table S1: Clinico-pathological data of the study cohort