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Applicability of a yeast bioassay in the detection of steroid esters in hair
Applicability of a yeast bioassay in the detection of steroid esters in hair
- Source :
- Analytical and Bioanalytical Chemistry 399 (2011) 3, Analytical and Bioanalytical Chemistry, 399(3), 1031-1039
- Publication Year :
- 2011
-
Abstract
- The aim of the present study was to demonstrate the applicability of a yeast androgen and estrogen bioassay in the detection of steroid esters in hair samples of animals treated with a hormone ester cocktail. The outcome of the activity screenings was critically compared with the results previously obtained with LC-MS/MS analysis. Hair samples of one pour-on treated animal, 10 ml DMSO containing 25 mg estradiol benzoate (EB), 60 mg testosterone decanoate (TD) and 60 mg testosterone cypionate (TC), were selected and analyzed with the androgen and estrogen yeast bioassay. Results showed that by the introduction of a hydrolysis step, bioassays can be used to screen for the presence of hormone esters in hair samples. Based on the difference in fluorescence responses between the nonhydrolyzed and the hydrolyzed hair samples, it was possible to detect the presence of EB up to at least 56 days after a single pour-on treatment and to detect the presence of TC and TD up to at least 14 days after the treatment. Although the LC-MS/MS analysis could detect TC and TD up to 49 days after treatment, bioassays have the advantage that they can also detect any (un)known steroid ester. Keywords Testosterone ester . Estradiol benzoate . Yeast bioassay . Untargeted analysis . Hair
- Subjects :
- green fluorescent protein
medicine.drug_class
medicine.medical_treatment
Fluorescence spectrometry
Biochemistry
Analytical Chemistry
Steroid
chemistry.chemical_compound
estradiol benzoate
Tandem Mass Spectrometry
Yeasts
expression
medicine
Animals
Bioassay
tandem mass-spectrometry
Testosterone
anabolic-steroids
samples
estrogenic activity
validation
Chromatography
Chemistry
RIKILT - R&C Groeibevorderaars
Organic Chemistry
calf urine
RIKILT B&T Authenticiteit en Nutrienten
Androgen
Organische Chemie
Estrogen
Estradiol benzoate
Biological Assay
Cattle
bovine hair
Testosterone decanoate
Rikilt B&T Toxicologie en Effectanalyse
Chromatography, Liquid
Hair
Hormone
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 16182642
- Database :
- OpenAIRE
- Journal :
- Analytical and Bioanalytical Chemistry 399 (2011) 3, Analytical and Bioanalytical Chemistry, 399(3), 1031-1039
- Accession number :
- edsair.doi.dedup.....8196a2fbdf0a8ceb46c7536b7f3e5e8a