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Apolipoprotein-mediated lipid antigen presentation in B cells provides a pathway for innate help by NKT cells
- Source :
- Blood. 114:2411-2416
- Publication Year :
- 2009
- Publisher :
- American Society of Hematology, 2009.
-
Abstract
- Natural killer T (NKT) cells are innate-like lymphocytes that recognize lipid antigens and have been shown to enhance B-cell activation and antibody production. B cells typically recruit T-cell help by presenting internalized antigens recognized by their surface antigen receptor. Here, we demonstrate a highly efficient means whereby human B cells present lipid antigens to NKT cells, capturing the antigen using apolipoprotein E (apoE) and the low-density lipoprotein receptor (LDL-R). ApoE dramatically enhances B-cell presentation of alpha-galactosylceramide (αGalCer), an exogenous CD1d presented antigen, inducing activation of NKT cells and the subsequent activation of B cells. B cells express the LDL-R on activation, and the activation of NKT cells by B cells is completely LDL-R dependent, as shown by blocking experiments and the complete lack of presentation when using apoE2, an isoform of apoE incapable of LDL-R binding. The dependence on apoE and the LDL-R is much more pronounced in B cells than we had previously seen in dendritic cells, which can apparently use alternate pathways of lipid antigen uptake. Thus, B cells use an apolipoprotein-mediated pathway of lipid antigen presentation, which constitutes a form of innate help for B cells by NKT cells.
- Subjects :
- Immunology
B-cell receptor
Antigen presentation
Naive B cell
CD1
Galactosylceramides
Lymphocyte Activation
Biochemistry
Apolipoproteins E
Humans
Antigen-presenting cell
Cells, Cultured
Antigen Presentation
B-Lymphocytes
CD40
biology
Antigen processing
Cell Biology
Hematology
Flow Cytometry
Cell biology
B-1 cell
Receptors, LDL
biology.protein
Natural Killer T-Cells
lipids (amino acids, peptides, and proteins)
Antigens, CD1d
Signal Transduction
Subjects
Details
- ISSN :
- 15280020 and 00064971
- Volume :
- 114
- Database :
- OpenAIRE
- Journal :
- Blood
- Accession number :
- edsair.doi.dedup.....81b4daefe272fa52f462770b0ff40bdd