Nonlinear Fourier-Transform Spectroscopy Using Ultrabroadband Femtosecond Pulses for the Measurement of Photobleaching of Fluorescent Proteins

Authors :: Keisuke Toda
Akira Suda
Hiroshi Takahashi
Source :: Springer Proceedings in Physics ISBN: 9783319132419
Publication Year :: 2015
Publisher :: Springer International Publishing, 2015.
Abstract: We examine the mechanism of photobleaching of fluorescent proteins using nonlinear Fourier-transform spectroscopy with ultrabroadband femtosecond pulses. Photobleaching of two-photon excited fluorescent molecules occurs through one-photon excited-state absorption.

Subjects :: Materials science
Spatial light modulator
business.industry
Physics::Optics
Photobleaching
Fluorescence
Quantitative Biology::Cell Behavior
Quantitative Biology::Subcellular Processes
symbols.namesake
Fourier transform
Optics
Excited state
Femtosecond
symbols
Spectroscopy
Absorption (electromagnetic radiation)
business

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