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Immediate translation of Formin DIAPH1 mRNA after its exiting the nucleus is required for its perinuclear localization in fibroblasts
- Source :
- PLoS ONE, PLoS ONE, Vol 8, Iss 6, p e68190 (2013)
- Publication Year :
- 2013
-
Abstract
- DIAPH1 is a formin protein which promotes actin polymerization, stabilizes microtubules and consequently is involved in cytoskeleton dynamics, cell migration and differentiation. In contrast to the relatively well-understood signaling cascades that regulate DIAPH1 activity, its spatial regulation of biogenesis is not understood. A recent report showed that synthesis of DIAPH1 is confined in the perinuclear ER compartment through translation-dependent mRNA targeting. However, the underlying mechanism of DIAPH1 local synthesis is yet to be elucidated. Here, we provide evidence to demonstrate that the 5'-cap-mediated immediate translation of DIAPH1 mRNA upon exiting nucleus is required for localizing the mRNA in the perinuclear ER compartment. This is supported by data: 1) Delayed translation of DIAPH1 mRNA resulted in loss of perinuclear localization of the mRNA; 2) Once delocalized, DIAPH1 mRNA could not be retargeted to the perinuclear region; and 3) The translation of DIAPH1 mRNA is 5'-cap dependent. These results provide new insights into the novel mechanism of DIAPH1 local synthesis. In addition, these findings have led to the development of new approaches for manipulating DIAPH1 mRNA localization and local protein synthesis in cells for functional studies. Furthermore, a correlation of DIAPH1 mRNA and DIAPH1 protein localization has been demonstrated using a new method to quantify the intracellular distribution of protein.
- Subjects :
- Fetal Proteins
lcsh:Medicine
Gene Expression
Chick Embryo
Protein Synthesis
Endoplasmic Reticulum
Biochemistry
03 medical and health sciences
0302 clinical medicine
P-bodies
Molecular Cell Biology
Protein biosynthesis
Genetics
Animals
RNA, Messenger
RNA synthesis
lcsh:Science
Cytoskeleton
Biology
030304 developmental biology
Adaptor Proteins, Signal Transducing
0303 health sciences
Multidisciplinary
biology
Endoplasmic reticulum
lcsh:R
Proteins
Translation (biology)
Fibroblasts
Protein subcellular localization prediction
Cellular Structures
Cell biology
Transport protein
Nucleic acids
Protein Transport
Cytoskeletal Proteins
Subcellular Organelles
Formins
Protein Biosynthesis
biology.protein
RNA
lcsh:Q
Protein Translation
RNA transport
030217 neurology & neurosurgery
Research Article
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....81c22a0c0787797f891daa1a7ecf6f97