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NUPR1 maintains autolysosomal efflux by activating SNAP25 transcription in cancer cells
- Publication Year :
- 2017
- Publisher :
- Taylor & Francis, 2017.
-
Abstract
- In the advanced stages of cancer, autophagy is thought to promote tumor progression through its ability to mitigate various cellular stresses. However, the details of how autophagy is homeostatically regulated in such tumors are unknown. Here, we report that NUPR1 (nuclear protein 1, transcriptional regulator), a transcriptional coregulator, is aberrantly expressed in a subset of cancer cells and predicts low overall survival rates for lung cancer patients. NUPR1 regulates the late stages of autolysosome processing through the induction of the SNARE protein SNAP25, which forms a complex with the lysosomal SNARE-associated protein VAMP8. NUPR1 depletion deregulates autophagic flux and impairs autolysosomal clearance, inducing massive cytoplasmic vacuolization and premature senescence in vitro and tumor suppression in vivo. Collectively, our data show that NUPR1 is a potent regulator of autolysosomal dynamics and is required for the progression of some epithelial cancers.
- Subjects :
- 0301 basic medicine
Lung Neoplasms
Synaptosomal-Associated Protein 25
Autolysosome
Autophagy
Regulator
SNAP25
Research Papers - Basic Science
Cell Biology
Biology
Cell biology
Neoplasm Proteins
Gene Expression Regulation, Neoplastic
03 medical and health sciences
030104 developmental biology
Tumor progression
Cancer cell
Transcriptional regulation
Basic Helix-Loop-Helix Transcription Factors
Humans
Nuclear protein
Lysosomes
Molecular Biology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....81f148f695658da7a86d4ad111f6de02