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Structures of B. subtilis Maturation RNases Captured on 50S Ribosome with Pre-rRNAs
- Source :
- Mol Cell, Molecular Cell, Molecular Cell, Elsevier, 2020, 80 (2), pp.227-236.e5. ⟨10.1016/j.molcel.2020.09.008⟩
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- International audience; The pathways for ribosomal RNA (rRNA) maturation diverge greatly among the domains of life. In the Gram-positive model bacterium, Bacillus subtilis, the final maturation steps of the two large ribosomal subunit (50S) rRNAs, 23S and 5S pre-rRNAs, are catalyzed by the double-strand specific ribonucleases (RNases) Mini-RNase III and RNase M5, respectively. Here we present a protocol that allowed us to solve the 3.0 and 3.1 Å resolution cryoelectron microscopy structures of these RNases poised to cleave their pre-rRNA substrates within the B. subtilis 50S particle. These data provide the first structural insights into rRNA maturation in bacteria by revealing how these RNases recognize and process double-stranded pre-rRNA. Our structures further uncover how specific ribosomal proteins act as chaperones to correctly fold the pre-rRNA substrates and, for Mini-III, anchor the RNase to the ribosome. These r-proteins thereby serve a quality-control function in the process from accurate ribosome assembly to rRNA processing.
- Subjects :
- Models, Molecular
RNase P
cryo-electron microscopy
Ribosome Subunits, Large, Bacterial
Biology
Ribosome
Article
Ribosome assembly
Substrate Specificity
03 medical and health sciences
0302 clinical medicine
Ribonucleases
Bacterial Proteins
Ribosomal protein
M5
Large ribosomal subunit
B. subtilis
RNA Precursors
RNase
structure
RRNA processing
Molecular Biology
030304 developmental biology
50S
0303 health sciences
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM]
Base Sequence
maturation
Cryoelectron Microscopy
Cell Biology
Ribosomal RNA
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Mini-III
Biochemistry
ribosome
RNA
030217 neurology & neurosurgery
Bacillus subtilis
Subjects
Details
- ISSN :
- 10972765
- Database :
- OpenAIRE
- Journal :
- Mol Cell, Molecular Cell, Molecular Cell, Elsevier, 2020, 80 (2), pp.227-236.e5. ⟨10.1016/j.molcel.2020.09.008⟩
- Accession number :
- edsair.doi.dedup.....81f17f648c6ac198d7e097f79b013c84