Back to Search
Start Over
A Phytophthora sojae G protein alpha subunit is involved in chemotaxis to soybean isoflavones
- Source :
- Eukaryotic Cell 7 (2008) 12, Eukaryotic Cell, 7(12), 2133-2140
- Publication Year :
- 2008
-
Abstract
- For the soybean pathogen Phytophthora sojae , chemotaxis of zoospores to isoflavones is believed to be critical for recognition of the host and for initiating infection. However, the molecular mechanisms underlying this chemotaxis are largely unknown. To investigate the role of G-protein and calcium signaling in chemotaxis, we analyzed the expression of several genes known to be involved in these pathways and selected one that was specifically expressed in sporangia and zoospores but not in mycelium. This gene, named PsGPA1 , is a single-copy gene in P. sojae and encodes a G-protein α subunit that shares 96% identity in amino acid sequence with that of Phytophthora infestans . To elucidate the function, expression of PsGPA1 was silenced by introducing antisense constructs into P. sojae. PsGPA1 silencing did not disturb hyphal growth or sporulation but severely affected zoospore behavior, including chemotaxis to the soybean isoflavone daidzein. Zoospore encystment and cyst germination were also altered, resulting in the inability of the PsGPA1 -silenced mutants to infect soybean. In addition, the expressions of a calmodulin gene, PsCAM1 , and two calcium- and calmodulin-dependent protein kinase genes, PsCMK3 and PsCMK4 , were increased in the mutant zoospores, suggesting that PsGPA1 negatively regulates the calcium signaling pathways that are likely involved in zoospore chemotaxis.
- Subjects :
- Hyphal growth
Phytophthora
Spores
signal-transduction
Zoospore
Protein subunit
Mutant
Molecular Sequence Data
cyst germination
Gene Dosage
Gene Expression
hyphal growth
Microbiology
oomycete
Phytophthora sojae
Calcium Signaling
Molecular Biology
Gene
transcription factor
Plant Diseases
zoospore encystment
biology
Virulence
EPS-2
Chemotaxis
Algal Proteins
fungi
food and beverages
General Medicine
Articles
biology.organism_classification
Isoflavones
GTP-Binding Protein alpha Subunits
Laboratorium voor Phytopathologie
Biochemistry
Phytophthora infestans
Laboratory of Phytopathology
beta-subunit
saccharomyces-cerevisiae
Soybeans
botrytis-cinerea
infestans
Subjects
Details
- Language :
- English
- ISSN :
- 15359778
- Volume :
- 7
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Eukaryotic Cell
- Accession number :
- edsair.doi.dedup.....820d6145ab398b7e329f9f7d060ddfc8