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Effects of β-estradiol on cold-sensitive receptor channel TRPM8 in ovariectomized rats
- Source :
- Experimental Animals
- Publication Year :
- 2017
- Publisher :
- Japanese Association for Laboratory Animal Science, 2017.
-
Abstract
- Transient receptor potential cation channel subfamily M member 8 (TRPM8) is associated with sensitivity to cold sensation in mammals. A previous study demonstrated that TRPM8 was overexpressed in the skin of ovariectomized (OVX) rats due to the loss of estrogen. In the present study, we investigated whether estrogen replacement restricts overexpression of the TRPM8 channel in the skin of OVX rats. We divided 15 Sprague Dawley rats into three groups: a non-operated group (NON-OPE), an ovariectomy group (OVX), and a group subjected to estrogen replacement during 4 weeks beginning 7 days after ovariectomy (OVX + E2). Five weeks later, TRPM8 channel mRNA and protein in lumbar skin were quantified by real-time RT-PCR, protein ELISA, and immunohistochemistry. The OVX + E2 group exhibited a trend for decreased expression of the TRPM8 channel in the lumbar skin in comparison with the OVX group, whereas ELISA data and immunohistochemistry data and immunohistochemistry graphs relating to TRPM8 protein did not show any obvious differences between the OVX group and the OVX + E2 group. Estrogen replacement may restrict the overexpression of TRPM8 in the dermis of OVX rats.
- Subjects :
- 0301 basic medicine
endocrine system
medicine.medical_specialty
Original
medicine.drug_class
Ovariectomy
Sensation
Gene Expression
TRPM Cation Channels
General Biochemistry, Genetics and Molecular Biology
Rats, Sprague-Dawley
03 medical and health sciences
Dermis
Internal medicine
estrogen
polycyclic compounds
medicine
TRPM8
Animals
Estrogen replacement
RNA, Messenger
oversensitivity to cold
Skin
estrogen replacement
Messenger RNA
Estradiol
General Veterinary
Chemistry
Estrogen Replacement Therapy
General Medicine
Cold Temperature
Postmenopause
Receptor channel
surgical procedures, operative
030104 developmental biology
Endocrinology
medicine.anatomical_structure
post-menopause
Estrogen
Ovariectomized rat
Immunohistochemistry
Female
Animal Science and Zoology
hormones, hormone substitutes, and hormone antagonists
cold-sensitive TRPM8 channel receptor
Subjects
Details
- ISSN :
- 18817122 and 13411357
- Volume :
- 66
- Database :
- OpenAIRE
- Journal :
- Experimental Animals
- Accession number :
- edsair.doi.dedup.....82177f786a94c81de73343789d05a087