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Syntaxin 5 regulates the endoplasmic reticulum channel-release properties of polycystin-2
- Source :
- Proceedings of the National Academy of Sciences of the United States of America. 105(41)
- Publication Year :
- 2008
-
Abstract
- Polycystin-2 (PC2), the gene product of one of two genes mutated in dominant polycystic kidney disease, is a member of the transient receptor potential cation channel family and can function as intracellular calcium (Ca 2+ ) release channel. We performed a yeast two-hybrid screen by using the NH 2 terminus of PC2 and identified syntaxin-5 (Stx5) as a putative interacting partner. Coimmunoprecipitation studies in cell lines and kidney tissues confirmed interaction of PC2 with Stx5 in vivo. In vitro binding assays showed that the interaction between Stx5 and PC2 is direct and defined the respective interaction domains as the t-SNARE region of Stx5 and amino acids 5 to 72 of PC2. Single channel studies showed that interaction with Stx5 specifically reduces PC2 channel activity. Epithelial cells overexpressing mutant PC2 that does not bind Stx5 had increased baseline cytosolic Ca 2+ levels, decreased endoplasmic reticulum (ER) Ca 2+ stores, and reduced Ca 2+ release from ER stores in response to vasopressin stimulation. Cells lacking PC2 altogether had reduced cytosolic Ca 2+ levels. Our data suggest that PC2 in the ER plays a role in cellular Ca 2+ homeostasis and that Stx5 functions to inactivate PC2 and prevent leaking of Ca 2+ from ER stores. Modulation of the PC2/Stx5 interaction may be a useful target for impacting dysregulated intracellular Ca 2+ signaling associated with polycystic kidney disease.
- Subjects :
- endocrine system
TRPP Cation Channels
Biology
Endoplasmic Reticulum
Calcium in biology
Mice
Polycystic kidney disease
medicine
Syntaxin
Animals
Homeostasis
Calcium Signaling
education
Calcium signaling
education.field_of_study
Multidisciplinary
Qa-SNARE Proteins
Endoplasmic reticulum
Epithelial Cells
Biological Sciences
medicine.disease
Cell biology
Cytosol
Polycystin 2
Calcium
Mutant Proteins
Intracellular
Protein Binding
Subjects
Details
- ISSN :
- 10916490
- Volume :
- 105
- Issue :
- 41
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Accession number :
- edsair.doi.dedup.....828f340d9b66bd0887ca4fda3ab80b71