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Identification and Expression of Neurotrophin-6 in the Brain of Nothobranchius furzeri: One More Piece in Neurotrophin Research

Authors :
Marina Paolucci
Chiara Attanasio
Antonio Palladino
Alessandro Cellerino
Paolo de Girolamo
Carla Lucini
Adele Leggieri
Eva Terzibasi Tozzini
Livia D'Angelo
Leggieri, Null
Attanasio, Null
Palladino, Null
Cellerino, Alessandro
Lucini, Null
Paolucci, Null
Terzibasi Tozzini, Eva
de Girolamo, Null
D’Angelo, Null
Leggieri, Adele
Attanasio, Chiara
Palladino, Antonio
Lucini, Carla
Paolucci, Marina
de Girolamo, Paolo
D'Angelo, Livia
Source :
Journal of Clinical Medicine, Journal of Clinical Medicine, Vol 8, Iss 5, p 595 (2019), Journal of Clinical Medicine; Volume 8; Issue 5; Pages: 595
Publication Year :
2019

Abstract

Neurotrophins contribute to the complexity of vertebrate nervous system, being involved in cognition and memory. Abnormalities associated with neurotrophin synthesis may lead to neuropathies, neurodegenerative disorders and age-associated cognitive decline. The genome of teleost fishes contains homologs of some mammalian neurotrophins as well as a gene coding for an additional neurotrophin (NT-6). In this study, we characterized this specific neurotrophin in the short-lived fish Nothobranchius furzeri, a relatively new model for aging studies. Thus, we report herein for the first time the age-related expression of a neurotrophin in a non-mammalian vertebrate. Interestingly, we found comparable expression levels of NT-6 in the brain of both young and old animals. More in detail, we used a locked nucleic acid probe and a riboprobe to investigate the neuroanatomical distribution of NT-6 mRNA revealing a significant expression of the neurotrophin in neurons of the forebrain (olfactory bulbs, dorsal and ventral telencephalon, and several diencephalic nuclei), midbrain (optic tectum, longitudinal tori, and semicircular tori), and hindbrain (valvula and body of cerebellum, reticular formation and octavolateral area of medulla oblongata). By combining in situ hybridization and immunohistochemistry, we showed that NT-6 mRNA is synthesized in mature neurons. These results contribute to better understanding the evolutionary history of neurotrophins in vertebrates, and their role in the adult brain.

Details

ISSN :
20770383
Volume :
8
Issue :
5
Database :
OpenAIRE
Journal :
Journal of clinical medicine
Accession number :
edsair.doi.dedup.....829dafacf35d111fc2cdcdb9bc9c1bc5