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Salivary diagnostics on paper microfluidic devices and their use as wearable sensors for glucose monitoring
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Microfluidic paper-based devices (μPADs) and wearable devices have been highly studied to be used as diagnostic tools due to their advantages such as simplicity and ability to provide instrument-free fast results. Diseases such as periodontitis and diabetes mellitus can potentially be detected through these devices by the detection of important biomarkers. This study describes the development of μPADs through craft cutter printing for glucose and nitrite salivary diagnostics. In addition, the use of μPADs integrated into a mouthguard as a wearable sensor for glucose monitoring is also presented. μPADs were designed to contain two detection zones for glucose and nitrite assays and a sampling zone interconnected by microfluidic channels. Initially, the analytical performance of the proposed μPADs was investigated and it provided linear behavior (r2 ≥ 0.994) in the concentration ranges between 0 to 2.0 mmol L−1 and 0 to 400 μmol L−1 for glucose and nitrite, respectively. Under the optimized conditions, the limits of detection achieved for glucose and nitrite were 27 μmol L−1 and 7 μmol L−1, respectively. Human saliva samples were collected from healthy individuals and patients previously diagnosed with periodontitis or diabetes and then analyzed on the proposed μPADs. The results found using μPADs revealed higher glucose concentration values in saliva collected from patients diagnosed with diabetes mellitus and greater nitrite concentrations in saliva collected from patients diagnosed with periodontitis, as expected. The results obtained on μPADs did not differ statistically from those measured by spectrophotometry. With the aim of developing paper-based wearable sensors, μPADs were integrated, for the first time, into a silicone mouthguard using a 3D-printed holder. The proof of concept was successfully demonstrated through the monitoring of the glucose concentration in saliva after the ingestion of chocolate. According to the results reported herein, paper-based microfluidic devices offer great potential for salivary diagnostics, making their integration into a silicone mouthguard possible, generating simple, low-cost, instrument-free, and powerful wearable sensors.
- Subjects :
- Paper
Saliva
business.product_category
Microfluidics
Wearable computer
02 engineering and technology
Diagnostic tools
Proof of Concept Study
01 natural sciences
Biochemistry
Analytical Chemistry
Wearable Electronic Devices
Limit of Detection
Diabetes Mellitus
Humans
Medicine
Mouthguard
Periodontitis
Nitrites
Wearable technology
business.industry
PERIODONTITE
010401 analytical chemistry
Reproducibility of Results
Microfluidic Analytical Techniques
021001 nanoscience & nanotechnology
0104 chemical sciences
Glucose
Salivary diagnostics
Case-Control Studies
Healthy individuals
Colorimetry
0210 nano-technology
business
Biomedical engineering
Subjects
Details
- ISSN :
- 16182650 and 16182642
- Volume :
- 411
- Database :
- OpenAIRE
- Journal :
- Analytical and Bioanalytical Chemistry
- Accession number :
- edsair.doi.dedup.....82b307f78293e8a79988f80a8babad24