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Magnetic Beads Based Immunoaffinity Capillary Electrophoresis of Total Serum IgE with Laser-Induced Fluorescence Detection
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Abstract
- A magnetic beads based immunoaffinity capillary electrophoresis method for total Immunoglobulin E quantification in serum has been developed. The method combines speed, automation ability, and minimal sample consumption. Only 1 microL of serum is required while the whole immunoaffinity capillary electrophoresis method is performed in less than 50 min. The concomitant use of online immunocapture, transient isotachophoresis, and laser-induced fluorescence detection provides a sensitivity in the low picomolar range and a highly linear fluorescence response over 4 orders of magnitude (IgE concentration ranging from 2.4 to 2400 ng/mL). After validation with a reference material, the method has been successfully applied to the quantification of total IgEs in patient sera. The results compared well with classical ImmunoCap data.
- Subjects :
- Immunoassay
Chromatography
Chemistry
Lasers
Fluorescence spectrometry
Electrophoresis, Capillary
Immunoglobulin E
Fluorescence
Orders of magnitude (mass)
Chromatography, Affinity
Analytical Chemistry
Electrophoresis
Magnetics
Capillary electrophoresis
Feasibility Studies
Humans
Isotachophoresis
Laser-induced fluorescence
Quantitative analysis (chemistry)
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....82bb8055e7f5dfa7c4495f9ed46a7252