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Immunological features and efficacy of the recombinant subunit vaccine LTB-EMY162 against Echinococcus multilocularis metacestode
- Source :
- Applied microbiology and biotechnology. 102(5)
- Publication Year :
- 2017
-
Abstract
- Alveolar echinococcosis is a zoonotic disease caused by the infection of the larval stage Echinococcus multilocularis with worldwide distribution especially in the northwest China. It is important to develop a well-tolerated immunoprophylaxis against E. multilocularis for alveolar echinococcosis control. In this study, a prokaryotic expression system for recombinant immunogen LTB-EMY162 was established, and the immunological features, sensitized lymphocyte, IL-4/IFN-γ secreted, prophylactic effect, and therapeutic effect were also evaluated. Arctic Express (DE3) system, Ni2+-charged and molecular sieve chromatography were used to obtain a high-purity 29 kDa protein. The ELISA and lymphocyte proliferation assay showed that LTB-EMY162 induced high-titer specific IgG against EMY162 and E. multilocularis protoscoleces protein in BALB/c mice and promoted sensitized T lymphocyte cell proliferation, and LTB-EMY162 stimulated Th cell to secrete IL-4 and IFN-γ and induced a Th1/Th2 mixed type immunological response. We also found that LTB-EMY162 significantly inhibited the cysts formation by challenging with 1000 E. multilocularis protoscoleces. The growth of protoscoleces and cysts were also significantly decreased by treating with LTB-EMY162 in 1000 protoscoleces intraperitoneal injection therapeutic mice model. In conclusion, we have constructed a subunit vaccine LTB-EMY162 which has prevention and therapeutic effect against E. multilocularis infection.
- Subjects :
- 0301 basic medicine
Immunogen
Lymphocyte
T-Lymphocytes
Cell
Antibodies, Helminth
Biology
Echinococcus multilocularis
Lymphocyte Activation
Applied Microbiology and Biotechnology
law.invention
Microbiology
03 medical and health sciences
Interferon-gamma
Mice
0302 clinical medicine
law
medicine
Animals
Humans
Life Cycle Stages
Mice, Inbred BALB C
Vaccines, Synthetic
Cell growth
General Medicine
T lymphocyte
biology.organism_classification
Metacestode
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Antigens, Helminth
Vaccines, Subunit
Recombinant DNA
Female
Interleukin-4
Biotechnology
Subjects
Details
- ISSN :
- 14320614
- Volume :
- 102
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Applied microbiology and biotechnology
- Accession number :
- edsair.doi.dedup.....82e4a885c547c72b28dc9739cec81f07