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Interleukin-10 inhibits autonomous myelopoiesis in patients with myelofibrosis

Authors :
Klaus Lechner
Heinz Gisslinger
Eva Jäger
Leopold Öhler
Ulrich Jäger
Klaus Geissler
Source :
European Journal of Haematology. 95:239-243
Publication Year :
2015
Publisher :
Wiley, 2015.

Abstract

The spontaneous formation of colony-forming units granulocyte/macrophage (CFU-GM) in semisolid cultures has been shown to be due to the endogenous release of cytokines and/or to the hypersensitivity of cells against growth factors. We have reported that increased autonomous CFU-GM growth is an in vitro characteristic of myelofibrosis (MF) which may reflect aberrant hematopoiesis in vivo. Because of its cytokine synthesis-inhibiting action, we speculated that interleukin-10 (IL-10) may inhibit pathological overproduction of myeloid cells in MF by suppression of autonomous myelopoiesis. In this study, IL-10 significantly inhibited autonomous CFU-GM formation in vitro from peripheral blood mononuclear cells (PB MNC) in 10 of 11 patients with MF tested. In all patients, there was a mean inhibition of 69% ranging from 35% to 100%. Suppression of autonomous CFU-GM formation by IL-10 was dose dependent and reversible by the addition of anti-IL-10 antibodies. Our results indicate that IL-10 is a potentially useful molecule to affect aberrant myelopoiesis in patients with MF.

Details

ISSN :
09024441
Volume :
95
Database :
OpenAIRE
Journal :
European Journal of Haematology
Accession number :
edsair.doi.dedup.....82fade7cf256d6d158e34aadc5d15b25
Full Text :
https://doi.org/10.1111/ejh.12486