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Genome-Wide Dynamic Evaluation of the UV-Induced DNA Damage Response
- Source :
- G3: Genes|Genomes|Genetics, G3: Genes, Genomes, Genetics, Vol 10, Iss 9, Pp 2981-2988 (2020)
- Publication Year :
- 2020
- Publisher :
- Oxford University Press (OUP), 2020.
-
Abstract
- Genetic screens in Saccharomyces cerevisiae have allowed for the identification of many genes as sensors or effectors of DNA damage, typically by comparing the fitness of genetic mutants in the presence or absence of DNA-damaging treatments. However, these static screens overlook the dynamic nature of DNA damage response pathways, missing time-dependent or transient effects. Here, we examine gene dependencies in the dynamic response to ultraviolet radiation-induced DNA damage by integrating ultra-high-density arrays of 6144 diploid gene deletion mutants with high-frequency time-lapse imaging. We identify 494 ultraviolet radiation response genes which, in addition to recovering molecular pathways and protein complexes previously annotated to DNA damage repair, include components of the CCR4-NOT complex, tRNA wobble modification, autophagy, and, most unexpectedly, 153 nuclear-encoded mitochondrial genes. Notably, mitochondria-deficient strains present time-dependent insensitivity to ultraviolet radiation, posing impaired mitochondrial function as a protective factor in the ultraviolet radiation response.
- Subjects :
- Mitochondrial DNA
Saccharomyces cerevisiae Proteins
DNA Repair
Ultraviolet Rays
DNA damage
Saccharomyces cerevisiae
Mutant
QH426-470
DNA damage response
Genome
03 medical and health sciences
0302 clinical medicine
Genetics
ultraviolet radiation response
high-throughput screen
Molecular Biology
Gene
Genetics (clinical)
030304 developmental biology
0303 health sciences
biology
Mutant Screen Report
biology.organism_classification
Cell biology
Transfer RNA
030217 neurology & neurosurgery
DNA Damage
Genetic screen
Subjects
Details
- ISSN :
- 21601836
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- G3 Genes|Genomes|Genetics
- Accession number :
- edsair.doi.dedup.....82fd733c98d201f143b174e21a7bcc52