Back to Search
Start Over
High prevalence of Tropheryma whipplei in Lao kindergarten children
- Source :
- PLoS Neglected Tropical Diseases, Vol 9, Iss 2, p e0003538 (2015), PLoS Neglected Tropical Diseases, PLoS Neglected Tropical Diseases, Public Library of Science, 2015, 9 (UNSP e0003538), ⟨10.1371/journal.pntd.0003538⟩, PLoS Neglected Tropical Diseases, 2015, 9 (UNSP e0003538), ⟨10.1371/journal.pntd.0003538⟩
- Publication Year :
- 2015
- Publisher :
- Public Library of Science (PLoS), 2015.
-
Abstract
- Background Tropheryma whipplei is a bacterium commonly found in feces of young children in Africa, but with no data from Asia. We estimated the prevalence of T. whipplei carriage in feces of children in Lao PDR (Laos). Methods/Principal Findings Using specific quantitative real-time PCR, followed by genotyping for each positive specimen, we estimated the prevalence of T. whipplei in 113 feces from 106 children in Vientiane, the Lao PDR (Laos). T. whipplei was detected in 48% (51/106) of children. Those aged ≤4 years were significantly less frequently positive (17/52, 33%) than older children (34/54, 63%; p< 0.001). Positive samples were genotyped. Eight genotypes were detected including 7 specific to Laos. Genotype 2, previously detected in Europe, was circulating (21% of positive children) in 2 kindergartens (Chompet and Akad). Genotypes 136 and 138 were specific to Chompet (21% and 15.8%, respectively) whereas genotype 139 was specific to Akad (10.55%). Conclusions/Significance T. whipplei is a widely distributed bacterium, highly prevalent in feces of healthy children in Laos. Further research is needed to identify the public health significance of this finding.<br />Author Summary Tropheryma whipplei is a common bacterium carried in feces of young children. Here, using specific PCR, we estimated the prevalence of T. whipplei in 113 feces from 106 children in Vientiane, the Lao PDR (Laos). T. whipplei was detected in 48% (51/106) of children. Eight genotypes were detected, including 7 specific to Laos. Genotype 2, previously detected in Europe, was circulating (21% of positive children) in 2 kindergartens (Chompet and Akad). Genotypes 136 and 138 were specific to Chompet (21% and 15.8%, respectively), whereas genotype 139 was specific to Akad (10.55%). Long regarded as a rare bacterium, now we can affirm that T. whipplei is a widely distributed bacterium, highly prevalent in feces including those from children in Vientiane.
- Subjects :
- AFRICA
Veterinary medicine
SENEGAL
lcsh:Arctic medicine. Tropical medicine
Genotype
POLYMERASE-CHAIN-REACTION
lcsh:RC955-962
Tropheryma
Real-Time Polymerase Chain Reaction
DISEASE
SALIVA
Tropheryma whipplei
Feces
Prevalence
Humans
Child
Genotyping
High prevalence
Schools
biology
Whipple Disease
STOOL
lcsh:Public aspects of medicine
Public Health, Environmental and Occupational Health
Genetic Variation
lcsh:RA1-1270
biology.organism_classification
Virology
3. Good health
Infectious Diseases
Carriage
Laos
WHIPPELII DNA
BACTERIUM
Child, Preschool
Female
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19352735 and 19352727
- Volume :
- 9
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- PLoS Neglected Tropical Diseases
- Accession number :
- edsair.doi.dedup.....8318bfc592499a393eda5a5ea0130187