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E2 multimeric scaffold for vaccine formulation: immune response by intranasal delivery and transcriptome profile of E2-pulsed dendritic cells
- Source :
- BMC Microbiology (Online) (2016). doi:10.1186/s12866-016-0772-x, info:cnr-pdr/source/autori:Trovato M, Maurano F, D'Apice L, Costa V, Sartorius R, Cuccaro F, McBurney SP, Krebs SJ, Prisco A, Ciccodicola A, Rossi M, Haigwood NL, De Berardinis P./titolo:E2 multimeric scaffold for vaccine formulation: immune response by intranasal delivery and transcriptome profile of E2-pulsed dendritic cells./doi:10.1186%2Fs12866-016-0772-x/rivista:BMC Microbiology (Online)/anno:2016/pagina_da:/pagina_a:/intervallo_pagine:/volume, BMC Microbiology
- Publication Year :
- 2016
- Publisher :
- BioMed Central, London , Regno Unito, 2016.
-
Abstract
- BACKGROUND: The E2 multimeric scaffold represents a powerful delivery system able to elicit robust humoral and cellular immune responses upon systemic administrations. Here recombinant E2 scaffold displaying the third variable loop of HIV-1 Envelope gp120 glycoprotein was administered via mucosa, and the mucosal and systemic immune responses were analysed. To gain further insights into the molecular mechanisms that orchestrate the immune response upon E2 vaccination, we analysed the transcriptome profile of dendritic cells (DCs) exposed to the E2 scaffold with the aim to define a specific gene expression signature for E2-primed immune responses. RESULTS: The in vivo immunogenicity and the potential of E2 scaffold as a mucosal vaccine candidate were investigated in BALB/c mice vaccinated via the intranasal route. Fecal and systemic antigen-specific IgA antibodies, cytokine-producing CD4(+) and CD8(+) cells were induced assessing the immunogenicity of E2 particles via intranasal administration. The cytokine analysis identified a mixed T-helper cell response, while the systemic antibody response showed a prevalence of IgG1 isotype indicative of a polarized Th2-type immune response. RNA-Sequencing analysis revealed that E2 scaffold up-regulates in DCs transcriptional regulators of the Th2-polarizing cell response, defining a type 2 DC transcriptomic signature. CONCLUSIONS: The current study provides experimental evidence to the possible application of E2 scaffold as antigen delivery system for mucosal immunization and taking advantages of genome-wide approach dissects the type of response induced by E2 particles.
- Subjects :
- CD4-Positive T-Lymphocytes
0301 basic medicine
medicine.medical_treatment
CD8-Positive T-Lymphocytes
HIV Envelope Protein gp120
Antibodies, Viral
Immunoglobulin G
Transcriptome
Th2
Mice
Immunogenicity, Vaccine
0302 clinical medicine
Mucosal immunity
AIDS Vaccines
Mice, Inbred BALB C
Vaccines
Bone marrow-derived dendritic cells
Immunogenicity
Transcriptome profile
3. Good health
Cytokine
Cytokines
Female
Antibody
Research Article
Microbiology (medical)
E2 scaffold
RNA-Sequencing
Biology
Microbiology
03 medical and health sciences
Immune system
Adjuvants, Immunologic
medicine
Animals
Immunity, Mucosal
Administration, Intranasal
Dendritic Cells
Vaccine
Immunoglobulin A
030104 developmental biology
Immunology
biology.protein
Nasal administration
CD8
030215 immunology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- BMC Microbiology (Online) (2016). doi:10.1186/s12866-016-0772-x, info:cnr-pdr/source/autori:Trovato M, Maurano F, D'Apice L, Costa V, Sartorius R, Cuccaro F, McBurney SP, Krebs SJ, Prisco A, Ciccodicola A, Rossi M, Haigwood NL, De Berardinis P./titolo:E2 multimeric scaffold for vaccine formulation: immune response by intranasal delivery and transcriptome profile of E2-pulsed dendritic cells./doi:10.1186%2Fs12866-016-0772-x/rivista:BMC Microbiology (Online)/anno:2016/pagina_da:/pagina_a:/intervallo_pagine:/volume, BMC Microbiology
- Accession number :
- edsair.doi.dedup.....8358884a2a8f3624e986ce52adf35931