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Contribution of sucrose synthase, ADP-glucose pyrophosphorylase and starch synthase to starch synthesis in developing pea seeds
- Source :
- HAL, Plant, Cell and Environment, Plant, Cell and Environment, Wiley, 1997, 20, pp.1421-1430
- Publication Year :
- 1997
- Publisher :
- Wiley, 1997.
-
Abstract
- Using genetic variability existing amongst nine pea genotypes (Pisum sativum L.), the biochemical basis of sink strength in developing pea seeds was investigated. Sink strength was considered to be reflected by the rate of starch synthesis (RSS) in the embryo, and sink activity in the seed was reflected by the relative rate of starch synthesis (RRSS). These rates were compared to the activities of three enzymes of the starch biosynthetic pathway [sucrose synthase (Sus), ADP-glucose pyrophosphorylase and starch synthase] at three developmental stages during seed filling (25, 50 and 75% of the dry seed weight). Complete sets of data collected during seed filling for the nine genotypes showed that, for all enzyme activities (expressed on a protein basis), only Sus in the embryo and seed coat was linearly and significantly correlated to RRSS. The contribution of the three enzyme activities to the variability in RSS and RRSS was evaluated by multiple regression analysis for the first two developmental stages. Only Sus activity in the embryo could explain, at least in part, the significant variability observed for both the RSS and the RRSS at each developmental stage. We conclude that Sus activity is a reliable marker of sink activity in developing pea seeds.
- Subjects :
- 0106 biological sciences
Physiology
Starch
Glucose-1-Phosphate Adenylyltransferase
Plant Science
01 natural sciences
Pisum
03 medical and health sciences
chemistry.chemical_compound
Sativum
Food science
030304 developmental biology
[SDV.EE]Life Sciences [q-bio]/Ecology, environment
2. Zero hunger
0303 health sciences
biology
food and beverages
Carbohydrate
biology.organism_classification
[SDV.EE] Life Sciences [q-bio]/Ecology, environment
Biochemistry
chemistry
biology.protein
Sucrose synthase
Sink (computing)
Starch synthase
010606 plant biology & botany
Subjects
Details
- ISSN :
- 13653040 and 01407791
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Plant, Cell and Environment
- Accession number :
- edsair.doi.dedup.....839bf951a595465b6a64a64d06c417b7