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Transforming growth factor-β1 signaling promotes epithelial-mesenchymal transition-like phenomena, cell motility, and cell invasion in synovial sarcoma cells
- Source :
- PLoS ONE, PLoS ONE, Vol 12, Iss 8, p e0182680 (2017)
- Publication Year :
- 2017
- Publisher :
- Public Library of Science, 2017.
-
Abstract
- The epithelial-to-mesenchymal transition (EMT) and the reverse process (the mesenchymal-to-epithelial transition [MET]) have been shown to be associated with tumor cell invasion and metastasis in different carcinomas. The EMT and MET have recently been shown to play a key role in the pathogenic processes of sarcomas, which are completely different from those of carcinomas. However, the definitive roles of the EMT in the tumorigenesis of synovial sarcomas remain unknown. Here, we explored whether transforming growth factor (TGF)-β signaling, an important oncogenic event in synovial sarcoma, modulates tumor cell characteristics related to the EMT, such as cell adhesion, migration, invasion, and proliferation. Interestingly, we found that TGF-β1 induced tumor cell activation, resulting in a tendency to aggregate and biphasic-like features. TGF-β1 also caused downregulation of E-cadherin and subsequent upregulation of N-cadherin, Snail, and Slug, which are responsible for EMT-like phenomena and increased cell motility and invasion. To further investigate the roles of TGF-β1 in the EMT, we established a SW982 cell line with stable TGF-β1 inhibition viaSB431542.These cells exhibited significantly decreased motility, migration, and proliferation (P = 0.001). Taken together, our data demonstrated that alterations in the TGF-β1/Smad signaling pathway could regulate the expression of EMT-related factors and the EMT process, resulting in changes in tumor cell invasion, migration, and proliferation in synovial sarcoma cells. These results may provide a important insights into therapeutic interventions and contribute to the present understanding of tumor progression in patients.
- Subjects :
- 0301 basic medicine
Pathology
Cell signaling
Physiology
lcsh:Medicine
Gene Expression
SMAD
Signal transduction
Biochemistry
0302 clinical medicine
Endocrinology
Medicine and Health Sciences
Neoplasm Metastasis
lcsh:Science
Cultured Tumor Cells
Multidisciplinary
Sarcomas
Sarcoma Cells
Signaling cascades
Cell migration
Synovial sarcoma
Cell Motility
Oncology
030220 oncology & carcinogenesis
embryonic structures
Biological Cultures
Research Article
medicine.medical_specialty
Cell biology
Epithelial-Mesenchymal Transition
Cell Migration
Biology
Research and Analysis Methods
Transforming Growth Factor beta1
03 medical and health sciences
Sarcoma, Synovial
Growth Factors
DNA-binding proteins
medicine
Genetics
Humans
Gene Regulation
Neoplasm Invasiveness
Epithelial–mesenchymal transition
Cell adhesion
Differentiated Tumors
Biology and life sciences
Endocrine Physiology
Cell growth
lcsh:R
Cancers and Neoplasms
Proteins
Cell Cultures
medicine.disease
Regulatory Proteins
030104 developmental biology
TGF-beta signaling cascade
Tumor progression
Cancer research
lcsh:Q
Transforming growth factor
Developmental Biology
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 12
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....83a01bcea7f7016702cc38405a6b21eb