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Cell-free Entry of Human T-Cell Leukemia Virus Type 1 to Mouse Cells
- Source :
- Japanese Journal of Cancer Research : Gann
- Publication Year :
- 2001
- Publisher :
- Wiley, 2001.
-
Abstract
- Human T-cell leukemia virus type 1 (HTLV-1) is the etiologic agent for adult T-cell leukemia and HTLV-1-associated myelopathy / tropical spastic paraparesis. Recently we infected newborn mice by inoculating HTLV-1-producing human cells, and found that T-cells, B-cells and granulocytes were infected in vivo. To understand the mechanism of viral-cell interaction and the pathogenesis of HTLV-1 using the mouse model, it is important to clarify the cellular tropism using a cell-free HTLV-1 transmission system. We employed a highly transmissible cell-free HTLV-1 produced by a feline kidney cell line, c77, and studied the susceptibility of 9 kinds of mouse cell lines, EL4, RLm1, CTLL-2, J774.1, DA-1, Ba / F3, WEHI-3, NIH3T3 and B1, and two kinds of human cell lines, Molt-4 and Hut78. HTLV-1 proviral sequence was found by PCR in all 9 mouse cell lines as well as in 2 human cell lines and viral entry was blocked with sera from an HTLV-1 carrier and an adult T-cell leukemia patient. Unexpectedly, mouse cell lines EL4 and RLm1 and human cell lines Molt-4 and Hut78 showed similar efficiency for viral entry. These results suggest a wide distribution of HTLV-1 receptor in mouse cells.
- Subjects :
- Cancer Research
T-Lymphocytes
viruses
Retroviridae Proteins, Oncogenic
Mouse cell lines
Entry
Gene Products, gag
Biology
Antibodies, Viral
Polymerase Chain Reaction
gag Gene Products, Human Immunodeficiency Virus
Article
Virus
Cell Line
Mice
In vivo
Viral entry
hemic and lymphatic diseases
Cell‐free transmission
Tropical spastic paraparesis
medicine
Animals
Humans
Leukemia-Lymphoma, Adult T-Cell
Receptor
Tropism
Human T-lymphotropic virus 1
Cell-Free System
Human T‐cell leukemia virus type 1
Virion
virus diseases
3T3 Cells
medicine.disease
Virology
Leukemia
Oncology
Cell culture
Carrier State
DNA, Viral
Cats
Subjects
Details
- ISSN :
- 09105050
- Volume :
- 92
- Database :
- OpenAIRE
- Journal :
- Japanese Journal of Cancer Research
- Accession number :
- edsair.doi.dedup.....83c3ae72be110d62a5b803ef53e8f9c4