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Embryonic protein undernutrition by albumen removal programs the hepatic amino acid and glucose metabolism during the perinatal period in an avian model
- Source :
- Plos One 4 (9), . (2014), PLoS ONE, PLoS ONE, Public Library of Science, 2014, 9 (4), ⟨10.1371/journal.pone.0094902⟩, PLoS ONE, Vol 9, Iss 4, p e94902 (2014)
- Publication Year :
- 2014
-
Abstract
- Different animal models have been used to study the effects of prenatal protein undernutrition and the mechanisms by which these occur. In mammals, the maternal diet is manipulated, exerting both direct nutritional and indirect hormonal effects. Chicken embryos develop independent from the hen in the egg. Therefore, in the chicken, the direct effects of protein deficiency by albumen removal early during incubation can be examined. Prenatal protein undernutrition was established in layer-type eggs by the partial replacement of albumen by saline at embryonic day 1 (albumen-deprived group), compared to a mock-treated sham and a non-treated control group. At hatch, survival of the albumen-deprived group was lower compared to the control and sham group due to increased early mortality by the manipulation. No treatment differences in yolk-free body weight or yolk weight could be detected. The water content of the yolk was reduced, whereas the water content of the carcass was increased in the albumen-deprived group, compared to the control group, indicating less uptake of nutrients from the yolk. At embryonic day 16, 20 and at hatch, plasma triiodothyronine (T3), corticosterone, lactate or glucose concentrations and hepatic glycogen content were not affected by treatment. At embryonic day 20, the plasma thyroxine (T4) concentrations of the albumen-deprived embryos was reduced compared to the control group, indicating a decreased metabolic rate. Screening for differential protein expression in the liver at hatch using two-dimensional difference gel electrophoresis revealed not only changed abundance of proteins important for amino acid metabolism, but also of enzymes related to energy and glucose metabolism. Interestingly, GLUT1, a glucose transporter, and PCK2 and FBP1, two out of three regulatory enzymes of the gluconeogenesis were dysregulated. No parallel differences in gene expressions causing the differences in protein abundance could be detected pointing to post-transcriptional or post-translational regulation of the observed differences. ispartof: PLoS One vol:9 issue:4 pages:1-12 ispartof: location:United States status: published
- Subjects :
- Proteomics
Embryology
Eggs
[SDV]Life Sciences [q-bio]
Protein metabolism
Gene Expression
Chick Embryo
Biochemistry
Poultry
chemistry.chemical_compound
Tandem Mass Spectrometry
Molecular Cell Biology
Gene Regulatory Networks
Electrophoresis, Gel, Two-Dimensional
Amino Acids
2. Zero hunger
Glucose Transporter Type 1
Multidisciplinary
Triiodothyronine
biology
Glycogen
Proteomic Databases
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Regulation, Developmental
Agriculture
Animal Models
Fructose-Bisphosphatase
Liver
embryonic structures
Medicine
Female
Research Article
medicine.medical_specialty
Livestock
food.ingredient
Science
Carbohydrate metabolism
Research and Analysis Methods
Avian Proteins
Model Organisms
food
Albumins
Yolk
Internal medicine
Genetics
medicine
Animals
Humans
Poultry Diseases
Glucose transporter
Biology and Life Sciences
Computational Biology
Cell Biology
Survival Analysis
Disease Models, Animal
Thyroxine
Glucose
Endocrinology
Animals, Newborn
chemistry
Gluconeogenesis
Kwashiorkor
biology.protein
GLUT1
Chickens
Organism Development
Protein Abundance
Phosphoenolpyruvate Carboxykinase (ATP)
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- Plos One 4 (9), . (2014), PLoS ONE, PLoS ONE, Public Library of Science, 2014, 9 (4), ⟨10.1371/journal.pone.0094902⟩, PLoS ONE, Vol 9, Iss 4, p e94902 (2014)
- Accession number :
- edsair.doi.dedup.....83ea714bf9e9432a3f57bdbe5178bd62