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Hyperlipidemic versus healthy pancreases: a proteomic analysis using an animal model
- Source :
- IUBMB life. 62(10)
- Publication Year :
- 2010
-
Abstract
- Hyperlipidemia is associated with a variety of pancreatic diseases; however, the underlying pathophysiology and molecular mechanisms remain undefined. Here, we performed a comparative proteomic analysis of pancreatic tissue obtained from hyperlipidemic rats to identify proteins that may be involved in mediating hyperlipidemia-associated pancreatic injury. Rats were fed a high-fat diet to induce hyperlipidemia. Control rats were fed a diet with normal fat content. Pancreatic tissue samples were obtained after 6 or 12 weeks and comparative proteomic analysis, using gel electrophoresis and mass spectrometry, was conducted to identify proteins, the expression of which were altered in pancreases from hyperlipidemic compared with control rat pancreases. The expression levels of 3 of 13 proteins were significantly altered in pancreatic samples from hyperlipidemic rats. Alpha-amylase and arginase II were dysregulated by more than twofold. These modulations persisted in pancreatic tissue obtained from late-stage hyperlipidemic rats. The levels of alpha-amylase and arginase II were significantly altered in pancreases obtained from rats with hyperlipidemia. These enzymes may be putative biomarkers of hyperlipidemia-mediated pancreatic injury.
- Subjects :
- Male
Proteomics
medicine.medical_specialty
Clinical Biochemistry
Hyperlipidemias
Pancreatic alpha-Amylases
Biology
Biochemistry
Rats, Sprague-Dawley
Tandem Mass Spectrometry
Internal medicine
Hyperlipidemia
Genetics
medicine
Animals
Electrophoresis, Gel, Two-Dimensional
Obesity
Molecular Biology
Pancreas
Triglycerides
chemistry.chemical_classification
Gel electrophoresis
Two-dimensional gel electrophoresis
Arginase
Pancreatic Diseases
Cell Biology
medicine.disease
Pathophysiology
Rats
Enzyme
medicine.anatomical_structure
Endocrinology
chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Models, Animal
Pancreatic injury
Subjects
Details
- ISSN :
- 15216551
- Volume :
- 62
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- IUBMB life
- Accession number :
- edsair.doi.dedup.....8403e7c20b31e5b63535354c662bc54b