Supplementary Figure 3 from A Hexane Fraction of American Ginseng Suppresses Mouse Colitis and Associated Colon Cancer: Anti-inflammatory and Proapoptotic Mechanisms

PDF file - 2.6MB, Effect of whole AG extract and different fractions of AG on IFN-γ-induced iNOS expression. The murine macrophage cell line (ANA-1 cells) was incubated for 12 hr with No AG (media only), the whole AG extract (260 �g/ml), or the indicated AG Fraction (260 �g/ml), washed, then exposed to IFN-γ (100 U/ml) for 0, 2, 4 and 8 hrs. Cell lysates were analyzed by Western blot analysis. C+, indicates the positive control, which was an archived ANA-1 cell lysate previously induced by IFN-γ, and known to have iNOS induction. The treatment was repeated 3 times to ensure consistency.